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احسان دانش

احسان دانش
قلم یہاں تک پہنچا تھا کہ ایک اخبار میں اچانک اپنے عزیز اوردیرینہ دوست احسان دانش کے انتقال کی خبر نظر سے گزری توصدمہ ہوا۔ مرحوم ضلع مظفر نگر کے ایک قصبہ میں پیداہوئے، لاہور پہنچ کرمزدوری کی،اسی سے مزدوروں کے شاعربنے۔ بڑی شہرت اورمقبولیت پائی، تقسیم کے بعد مرجھا سے گئے تاہم اطمینان اورفارغ البالی کی زندگی بسر کرتے تھے۔ طبعاً مرنج ومرنجان،متواضع و منکسر المزاج اورعقیدہ وعمل کے اعتبار سے پکے مسلمان تھے۔
اللھم اغفرلہ وارحمہ۔
[اپریل۱۹۸۲ء]

 

پاکستانی نوجوان’’ فکری انحراف، اسباب اور سدباب‘‘ اسوہ حسنہ کی روشنی میں

The Pakistani youth are engaged day and night in a struggle for attainment of education, technology, and status. But what is deplorable is that they have forgotten their cultural values, ethics, code of life, and religious identity in order to unite with external powers in becoming part of the drive for development and they have become ignorant of their fundamental responsibilities as a member of the Muslim Ummah. What are the priorities and issues facing Muslims on the local, national, and international levels? Especially in Europe and America the Muslim youth are standing at the crossroads. They are undergoing a religious, ideological, and moral decline. History is eye witness to how the Muslim youth made valuable sacrifices in all walks of life and persevered in making incredible achievements. Moreover, it is the three-fold ideological, cultural, and emotional invasion of the anti-Islamic forces which has been the cause of a weakening of faith in the Muslim Ummah in general and the young generation in particular, since ideology is of primary significance for any nation, religion, movement, or group. It is true that nations are formed and sustained on the basis of ideology. The moment the ideological base is weakened, decline and dissolution become the fate of nations. They are unaware of how it is our foremost national duty and an urgent need to develop scholars who would propagate the Islamic agenda. Contrastively, the anti-Islamic forces are engaged in engendering their representatives. Hence, in order to safeguard our youth from ideological and religious dissolution it is necessary that educational and cultural steps are taken in society in advance so that our youth are provided with a wholesome environment free of ideological dissolution. An outline of the article is given below: The importance and significance of the prime of youth, The ideological propensities of the youth, The causes of dissension in youth, The remedy of dissension, are discussed in detail in this article.

Expression Profiling of Huanglongbing Disease in Citrus Though Molecular Techniques

Huanglongbing (HLB), also called greening, is a fatal disease of citrus. Candidatus Liberibacter, a Gram negative and non culturable bacterium, is the pathogen of huanglongbing. It becomes difficult to detect this bacterium because it is unevenly distributed in its citrus hosts.The bacteria are transmitted naturally to citrus by an insect vector called citrus psyllid. Hussain and Nath documented the occurrence of HLB and its related vector Diaphorina citri Kuwayama in Pakistan in 1927.In this study, 97 accessions of citrus germplasm were raised in greenhouse. of 97 accessions, 51 were survived. The seed of citrus germplasm was obtained from National Clonal Germplasm Repository for Citrus and Dates, Riverside, California, USA and University of Agriculture Faisalabad (UAF), Pakistan. For HLB diagnosis in the leaf samples of sweet orange (Citrus sinensis) cultivar Succari from field, conventional polymerase chain reaction technique was employed. Asian citrus psyllid (ACP) was captured from HLB positive sweet orange field trees and released on healthy plants of sweet orange and kinnow for rearing and infestation in the controlled conditions of growth room. Those plants were also tested for their HLB positivity by using 16s rDNA primers OI1/OI2c and Candidatus Liberibacter asiaticus specific primer A2/J5. After confirmation of HLB, sweet orange plants infested by ACP in growth room, were usedfor the inoculation of fifty one genotypes of citrus and citrus relatives. Candidatus Liberibacter asiaticus was detected in 96.07% of the inoculated citrus germplasm by Taqman based real time PCR using primers set HLBasfpr.SYBR green based real time qPCR was performed to differentiate expression of genes in HLB infected and healthy leaf samples of forty five genotypes of citrus and its relatives. Seven genes, includingsulfate transferase (CsSULF), glucose-1-phosphate adenyl transferase (CsSB1), granule bound starch synthase (CsSB2), alpha amylase (CsSD1), alpha amylase 3 (CsSD2), beta amylase9 (CsSD3) and cytochrome P450 mono oxygenase 83B1(CsSUR2) were tested for expression profiling. Gene expression data analysis for relative quantity represented the down regulation ofcarbohydrate metabolism related genes (CsSB1and CsSD3) in Clausena harmandiana while transporter gene CsSULF expressed at equal level in healthy and diseased Glycosmis pentaphylla. Remaining genes did not express in both of the varieties. These two genotypes may be placed in tolerant category against HLB as the causal bacterium was not detected in them. In Tahitian and Schuab, no gene was amplified except CsSULF gene that was down regulated indicating strong root system for plant survival.
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