مقدراں دی کھیڈ
ناہید اک وڈے سرکاری افسر دی اکلی اولاد سی۔ ایس لئی اوس دی پرورش بہت ای چنگے ڈھنگ نال ہوئی اوس نوں ماں تے پیو دا رجھواں پیار وی ملیا۔ جدوں اوس نے بی ایس سی دا امتحان دتا تاں اوس دے گھر کئی رشتے آنا شروع ہوگئے۔ اک دن ناہید دی ماں دی سہیلی اوہناں دے گھر آئی تے مینوں اپنے پتر اعجاز لئی منگ لیا۔ حالے اوہدا نتیجہ وی نئیں سی آیا کہ اوہ ناہید اعجاز بن گئی۔
اعجاز اک چنگا، سوہنا تے کماؤ پتر سی۔ گھر دے سارے جی ناہید دا خیال رکھدے سن۔ کیوں جے اعجاز اک سرکاری ملازم سی ایس لئی اک سال لنگھن دا پتہ وی نہ لگا۔ ویاہ توں اک سال بعد میں اک پتر دی دی ماں بن گئی۔ پتر دا ناں آصف رکھیا گیا۔ آصف دے جمن تے سارے گھر والے خاص کر سس سوہرا تے جیٹھانی بہت خوش سن۔ اوس دی وڈی وجہ ایہہ سی کہ میرے جیٹھ دی کوئی اولاد نئیں سی۔ سارے ای آصف نال انتہا دا پیار کردے سن۔
ایہناں دناں وچ بہت کم ہوون پاروں اعجاز بوہتا مصروف ہوگیا، کدے رات نوں چھیتی گھر...
Islam is the perfect religion from every aspect and for every time which provides guidelines for all situations of human life. Islamic political system is one of these aspects which is based on the principles of Holy Quran and sayings of the prophet Muhammad (PBUH), and it supports such a civil structure which follows the directives and requirements mentioned in the afore said two main sources. In Islamic political system, the supreme authority is Almighty Allah and human being utilizes this right as His vicegerent. The present global political system and the strategy of Muslims invite the attention of the thinkers around the globe for effective international interaction between Islamic world and others. This shows that serious deliberations are direly needed to compete with the current challenges. In the present paper, the contemporary situation of global political system will be discussed in order to determine the development of western political system, suggest means to compete with the challenges of this age and to facilitate in devising a suitable strategy for it
Successful repair of DNA double strand breaks is crucial; failure of which can lead to tumourigenesis including breast/ovarian cancer. BRCA2 (breast cancer susceptibility protein type 2) and RAD51 from different specie interact in a process called homologous recombination (HR) during the repair of such kind of insults to DNA. BRCA2 being a large protein molecule has different sites of interaction for other proteins like DMC1 (disruption of meiotic control 1). In the present study, we have purified a distinct region in human BRCA2 close to DMC1 binding site which has been shown to undergo CDK (cyclin dependent kinase) based phosphorylation in our kinase assays. Characterization involving dynamic light scattering, limited proteolysis and circular dichroism spectroscopy techniques revealed this region to be mostly disordered. We have also attempted the successful purification of DMC1 (different from the previous method of its purification) employing C-terminus of DMC1 as binding partner for increased solubility. Further studies included the characterization of Trypanosoma brucei BRC repeats (TbBRC) and Rad51 interaction. We observed no interaction of Trypanosoma brucei Rad51 (TbRad51) with its BRC repeats upto 7 BRC repeats based on our in vitro pull down assays. The comparison of human RAD51 and TbRad51 structures highlighted critical differences of residues at hydrophobic cavities for the binding of phenylalanine from BRC repeat. We predicted that TbBRC repeats, collectively, might have WD-40 β-propeller structure. The purification of Trypanosoma brucei 7 BRC repeats rendered this protein highly soluble and stable based on circular dichroism spectroscopy which also revealed the protein having poly-proline structure, a characteristic of unordered proteins. Moreover, we have predicted multiple phosphorylation sites in TbRad51 and compared them with other eukaryotic Rad51 orthologues to get insights into a possible regulatory mechanism for Rad51.