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جن خیالوں میں

جن خیالوں میں۔۔۔!
جن خیالوں میں اثبات درد کے ساتھ
سرخ دھڑکنوں کی تاثیر سمائی ہوئی ہے
وہ گمنام جزیروں سے۔۔۔
اک مہکتا ہوا خط لاتے ہوئے!
سوسن و نسترن میں زعفرانی گرھیں کھول کر!
چاہتوں کی میزان پر۔۔۔!
پہلی آہٹ۔۔۔پہلی فکر اور سوچ کو سوچتے ہوئے!
دشت میں اک تھکی ہاری ناقہ کے ساتھ،
تورات والانجیل جیسی غزالہ بھی لائی ہوئی ہے

جناب حسان، رواحہ اور جامی کے پاؤں چوم کر۔۔۔!
دریا کنارے مسافروں کو خیمہ زن کرتے ہوئے!
گل مریم کے کندھوں پر۔۔۔گل داؤدی کی گھٹڑی رکھتے ہوئے!
چاندنی کے ساتھ۔۔۔اک چراغ کا طواف کرنے آئی ہوئی ہے
تورات و انجیل جیسی غزالہ بھی لائی ہوئی ہے
پانیوں پر وجد طاری کرتے ہوئے!
محبتوں، چاہتوں کا صحیفہ تلاوت کرنے آئی ہوئی ہے
اک مہکتا ہوا خط بھی ساتھ لائی ہوئی ہے
تورات و انجیل جیسی غزالہ بھی لائی ہوئی ہے

منھج الشیخ السھارنفوری فی رفع الشبھات العقلیۃ: دراسۃ تحلیلیۃ فی ضوء ’’بذل المجھود‘‘ نموذج

Explanation of Hadith literature is a very significant academic contribution of Muhadditheen since the dawn of this sacred source. Sunan by Abu Dawod (d. 275 A.H.) has its well reputation in field Hadith codification and it has taken a perpetual attraction of Hadith scholars for its interpretation. Molana Saharanpuri (d.1927A.D.)is a famous sub continental Muslim scholar who contributed a voluminous interpretation titled ‘Bazl al-Majhood’ in which he comprehensively explores different aspect of Hadith. He has given an exploration of intellecttu-al solutions to various doubts and objection in very lucid way. The article has been rendered to focus on the same issue and intends to deal with the method-ology adopted by Saharanpuri while resolving the insinuations regarding Hadith literature.

Molecular Cloning and Overexpression of Heat Shock Transcription Factor Hsfa1d in Tobacco for Enhancement of Thermotolerance

Among abiotic stresses, heat stress has the most devastating impact on plant growth. In the present study, heat shock transcription factor HsfA1d was isolated from Arabidopsis thaliana and cloned into an in-house constructed gateway compatible cloning vector pUC57GW-CmRccdB through infusion cloning. Entry clone was confirmed through restriction digestion and sequencing analysis. For subcellular localization, HsfA1d was cloned in-frame with yellow florescent protein (YFP) in pGWB442 through LR clonase reaction resulted in YFP-HsfA1d chimeric gene construct. Homodimerization of HsfA1d was studied using Bimolecular Florescence complementation (BIFC) assay. For BIFC, HsfA1d was cloned in-frame with sequences that codes for N and C termini of yellow florescent protein, into 2 in-house constructed vectors pGSA002-nYFPn and pGSA002-nYFPc respectively. After confirmation through restriction enzyme digestion, the newly constructed vectors were transformed into Agrobacterium tumefaciens GV3101 for transient expression in tobacco (Nicotiana benthamiana) through syringe-infiltration. Subcellular localization of YFP-HsfA1d in DAPI-stained cells was evaluated using confocal microscopy. For plant transformation, HsfA1d was cloned into 2 plant expression vectors pGWB402Ω and pGWB442 through LR clonase reaction. After confirmation through RFLP analysis, the vectors were transformed into Agrobacterium GV3101 for tobacco leaf disc infection. Putative transgenic plants were selected and regenerated on appropriate selection media using different growth regulators. After PCR and confocal-based confirmation, transgenic plants were evaluated for their response to heat stress. HsfA1d, HSP70 and HSP90 exhibited 4.8, 2.8 and 2 folds increase respectively in their expression under heat stress compare to room temperature. HsfA1d was found to significantly enhance the expression of downstream gene HSP70 while no such effect on the expression of HSP90 was recorded. The plants transformed with HsfA1d were found to retain more water and accumulate more proline under heat stress. The transgenic plants exhibited efficient protective system, causing less 8 electrolyte leakage and less chlorophyll damage under heat stress. It is concluded that HsfA1d plays a vital role in thermotolerance enhancement and hence recommended for increasing plant thermotolerance.
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