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آہ! پی پی پی

آہ پی پی پی

کہتا ہے کون ختم ہوئی کربلا کی جنگ

ہم لڑ رہے ہیں آج بھی فوجِ یزید سے

آہ! پی پی پی

زخموں ،کوڑوں ،جیلوں ،پھانسیوں ،ڈنڈوں ،برچھیوں اور بھالوں کے وار سہنے کا ایک لامتناہی سلسلہ قیادت سے لے کر ایک ورکر ایک جیالے تک قربانیوں کی نہ ختم ہو نے والی داستان ۔

 

 

The Effect of Counseling and Training on Public Knowledge About Making Handsanitizer from Betel Extract

Hand sanitizers may help increase hand hygiene compliance as the results of invailability of basic needs for cleaning such as soap or water. Since alcohol has an irritating effect on the skin and burns quickly, it is currently discouraged as an active ingredient. Knowledge of covid 19 is critical for the world to have in order for individuals to be able to make informed choices on how to split the covid 19 transmission chain. This thesis employs a pre-experimental method that employs the One Group Pretest-Posttest Design approach. The paired t test was used to examine the relationship between the two variables, namely the betel leaf extract handsanitizer output preparation and the respondent's knowledge of the handsanitizer making process. Extension has an impact on increasing respondents' awareness of how to make natural hand sanitizers. The paired t-test findings indicate that the Sig. = 0.000 0.05, indicating that there is a discrepancy between the pretest and posttest. With a mean value of -5.967, this data indicates the average discrepancy between the pretest and posttest, with a range of -5.383 to -6.551 (95 percent CI), indicating that there is a difference between the pretest and posttest mean values, indicating that the pretest value has an influence on the post test. To stop the spread of Covid19, health care providers and the society must work together.

Pathological and Comparative Analysis of Newcastle Disease Virus of Sub-Genotypes Vi G and Vi M in Pigeons

Newcastle disease viruses (NDV) cause infection in avian species including both poultry and non-poultry worldwide. It is an extremely contagious disease also affecting vaccinated birds. Newcastle disease (ND) is endemic in Pakistan, viruses of sub-genotype VIIi in genotype VII, and sub-genotype VIg and Vim in genotypes VI of class II is continually effecting the pigeons and poultry production facilities. The identification and characterization of these viruses in poultry and non-poultry species is necessary in order to create realistic strategies for preventing and controlling NDV outbreaks. Genotype VI are also termed as pigeon paramyxoviryus-1, are frequently isolated and cause disease from the columbidae family. In this study, two PPMV-1 strains isolated from outbreaks in Lahore district were characterized by pathotypically, genotypically and detailed clinicopathologic assessment. The intracerebral pathogenicity index (ICPI) values for pigeon/Pak/Lahore/SA_1/2017 and pigeon/Pak/Lahore/SA_2/2017 were 1.51 and 1.52 respectively. The complete genome analysis based on full F gene coding sequences of two pigeon origin isolates from Lahore in 2017, along with previously published NDV strains available in GenBank was conducted. Analysis of F gene showed clustering of the isolates with genotype VI g and VI m. The genetic distance and phylogenetic analysis showed comparison with class II genotypes including vaccine strains. Both the strains were up to 10% genetically distant from LaSota (genotype II) isolate. Additionally, when compared for nucleotide and deduced amino acid sequence comparison several mutations were observed. The analysis of F and HN gene functional domains and receptor sites displayed conserved and mutated sequences. Results of genome analysis suggested that PPMV-1 isolates had antigenic variations from vaccine strain LaSota. For histopathological examination pigeon groups were challenged with pi/Lhr/SA_1/17 and pi/Lhr/SA_2/17 strains. Although severe clinical signs were not observed but microscopic results revealed lesions in multiple tissues from selected organs. Though limited data is available in Pakistan, this study will assist in introducing new control strategies and preventive measures for PPMV-1 outbreaks.
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