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حرف آغاز

ڈاکٹر نصیر احمد اسد کی تصنیف’’ ارضِ اقبال آ فاقیت کے آ ئینے میں‘‘ کے مقالات جہاں ان کے تحقیقی ذوق کے آ ئینہ دار ہیں وہیں ان کی تنقیدی بصیرت کے بھی گواہ ہیں۔ انہوں نے بنیادی مآخذ سے استفادہ کرتے ہوئے زیر نظر موضوعات کا نہایت تفصیل کے ساتھ جائزہ لیا ہے ۔
     ایک بہت بڑی خوبی جو ان مضامین میں از اوّل تا آ خر موجود ہے وہ یہ کہ اُنہوں نے محض مشاہیر ادب و تحقیق کی آ را اور فیصلوں کو نقل نہیں کیا بلکہ اپنا نقطہ نظر پیش کیا ہے۔ ان کا یہ طالب علمانہ انداز ان کے ان مضامین کو انفرادی رنگ کا حامل بناتا ہے۔ تاہم یہ کم خوبی کی بات نہیں کہ زیر نظر موضوع پر ریسرچ سکالرز کا اپنا نقطہ نظر سامنے آ تا ہے۔ان مضامین میں حواشی ،حوالہ جات اور تعلیقات کا خاص خیال رکھا گیا ہے۔ اس کے علاوہ مواد ، زبان و بیان اور پیش کش کے اعتبار سے یہ مضامین لائق مطالعہ اور قابلِ تحسین ہیں ۔
    تحقیقی وتنقیدی دنیا میں ڈاکٹر نصیر احمد اسد پہلی بار ’’ارضِ اقبال آفاقیت کے آ ئینے میں‘‘ کے ساتھ داخل ہو رہا ہے۔ ان کی یہ تازہ کاری،ذوق و شوق اور سعی و کاوش خوش آئند ہے۔ان کے علمی انہماک اور وفور شوق کے باعث امید کی جا سکتی ہے کہ وہ آ ئندہ میدان ادب و تحقیق میں اپنے کام کے معیار کو مزید بلندیوں سے ہم کنار کریں گے۔
ڈاکٹر یوسف خشک
چئیر مین اکادمی ادبیات اسلام آباد ، پاکستان

IMMEDIATE EFFECTS OF POST-ISOMETRIC RELAXATION AND STATIC STRETCHING ON QUADRICEPS MUSCLE DURING VERTICAL JUMP IN VOLLEY BALL AND BASKETBALL PLAYERS

Background and Aim: In today sports such as volleyball and basketball, Vertical Jump is considered as important component that enhance the performance of athlete. The aim of this study was to determine the effect of muscle energy technique on vertical jump performance in volleyball and basketball players. Methodology: This Randomized Clinical Trial was conducted at Islamic International University Islamabad. Twenty-nine athletes of age 18 – 35 playing basketball and volley as part time/domestic level were included. Post isometric relaxation technique was applied on group A (n=15) and static stretching was applied on group B (n=14). Surface electromyography activity of quadriceps and abdominal muscle was recorded, Stable time, airtime and vertical jump height were measured using two-axis force platform and vertical jump height was measured by motion sensor. Measurements were taken at baseline and immediately after applying interventions to both groups. Data entry and analysis were done by using software SPSS version 22. Results:   Of the 29 athletes, 15 were in the group A and 14 were in the group B. Immediate assessment of vertical jump height was not significantly improved by post-isometric relaxation relative to static stretching (p=0.594). Muscle recruitment, ground reaction and vertical jump height improved apparently after post isometric relaxation but not significantly. Conclusion: It appears that post isometric relaxation and static stretching of quadriceps shows no significant difference in vertical jump height.

Seroprevalence and Molecular Characterization of Infectious Bronchitis Virus Variants from Poultry in Pakistan

Infectious bronchitis virus (IBV) is incriminated in a variety of clinical conditions in poultry. IBV has a potential to mutate under field conditions and due to this multiple serotypes and variants of the IB virus circulates in commercial and backyard poultry. In the present study seroprevalence levels of different known serotypes of IBV such as, Mass-41, 4/91, D274, D1466, IT-02, D388 & D8880 were first determined in non IB-vaccinated poultry from different provinces of Pakistan during 2012 to 2015. The data showed high seroprevalence of multiple serotypes of IBV indicating high level of diversity in the circulating serotypes/variants of IBV in this country. In addition to this, a post-IBV vaccination base line was developed by sero-monitoring among healthy poultry in response to IBV vaccines, providing a permanent reference for future monitoring of post-vaccination antibody titers For further IBV investigations, different diagnostic techniques to be used in this study were optimized in this study. Moreover, studies regarding the determination of tissue tropism of one of the new IBV isolates along with evaluation of its co-infection potential with Avian Influenza virus H9N2 and ORT were also carried out. For the detection and typing of locally circulating Pak-IBV isolates following techniques were first optimized including, haemagglutination (HA), haemagglutination inhibition (HI), Indirect-Enzyme linked immunosorbent assay (ELISA), restriction fragment length polymorphism (RFLP), reverse transcriptase polymerase chain reaction (RT-PCR), real time reverse transcriptase polymerase chain reaction (QRT-PCR), virus neutralization (VN). Using these techniques, a total of 3187 clinical samples were processed for IBV detection, out of this 871 were IBV positive. Moreover, subtype detection revealed that 45.2% was Mass-41, 51.3% was IBV serotype 793-B and 3.4% were variants or un-identified. Furthermore, 871 RT-PCR positive samples were propagated upon in-ovo inoculation in specific pathogen free (SPF) embryonated chicken eggs. Out of this 55 IBV isolates were subjected to RFLP analysis that grouped the isolates into three segments, first was designated as 4/91 like group, second was designated as Mass-41 like group and the third was designated as IBV variant. The sequence and phylogenetic analysis of three IBV isolates from each RFLP group revealed that the first isolate, Pak IBV-786, shared sequence homology of the range of 25 99.1%-99.5% with 4/91 like strains from China, India, Russia, Morocco, Japan and Iran or GI-13 IBV lineage. The second isolate, Pak IBV-1113 shared 98% sequence of its sequence with IBV vaccine strains of Ma5 and M41 from Brazil, India, USA, Egypt, China, Iran, Thailand and Poland. The third and new Pak isolate, IBV-973, shared 91-93% of its sequence with the Indian strains of IBV earlier reported in India only, of GI-24 lineage. This strain of IBV variant has been first time reported from commercial poultry in Pakistan and its subsequent molecular characterization revealed that this virus is in fact a new serotype of IBV earlier only reported from India. Pathogenesis and co-infection studies on the isolate Pak-973 further highlighted biological characteristics of the new Pak-variant, which led us to believe that this variant may be contributing significantly towards the development of super complex of Respiratory Tract Infection, with or without the involvement of AIV H9N2. The sequence and phylogenetic analysis of different Pak IBV isolates reflected here that the genome of IBV is under a continuous process of evolution, due to point mutations, selective pressure (vaccine) and recombination events. So as like other RNA viruses, the IBV control is most likely to succeed upon using serotype specific vaccines (Homologous vaccines), as carried out elsewhere. It would, therefore be highly appropriate to recommend the incorporation of strain Pak-973 in commercial poultry vaccines being used in this country. INDEX WORDS: Infectious bronchitis virus, Pak-variants, Spike glycoprotein, RFLP, Real time RT-PCR, Co-infection, Tissue tropism
Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

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