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شیخ علی طنطاوی

شیخ علی طنطاوی
گزشتہ ماہ عربی زبان کے نام ور ادیب مشہور واعظ ومصلح علامہ شیخ علی طنطاوی کا انتقال ہوگیا، اناﷲ وااناالیہ راجعون۔
وہ کافی معمر اور ضعیف ہوچکے تھے۔ انتقال کے وقت ان کی عمر سو برس سے تجاوز تھی۔ مسجد حرام میں ان کی نماز جنازہ ادا کی گئی اور بلد حرام میں مدفون ہوئے۔
ان کی نسبت سے پتہ چلتا ہے کہ ان کا آبائی وطن طنطا مصر تھا، مگر وہ ایک مدت تک شام میں مقیم رہے اور وہاں منصب قضا پر بھی فائز رہے، شام میں فوجی انقلاب کے بعد غالباً وہ وہاں سے سعودیہ عربیہ منتقل ہوگئے اور عمر کا بقیہ حصہ یہیں بسر کیا۔
سعودی ریڈیو اور ٹیلی ویژن پر ان کی مذہبی تقریریں اور علمی سوال و جواب کا سلسلہ کافی مقبول ہوا۔ وہ عربی کے بلند پایہ ادیب اور انشا پر داز تھے، حضرت مولانا سید ابوالحسن علی ندوی مدظلہٰ سے ان کے گہرے علمی روابط تھے، چنانچہ مولانا کی کئی کتابوں پر انہوں نے پیش لفظ لکھا۔ دارالعلوم ندوۃالعلماء بھی تشریف لائے اور اس کے متعلق والہانہ جذبات کا اظہار کیا۔ ندوہ کے عربی رسالہ ’’البعث الاسلامی‘‘ میں ان کے مضامین برابر نقل کئے جاتے تھے۔
شیخ طنطادی کا خاص مشن عرب نوجوانوں کی مذہبی اصلاح تھا، وہ خاص طور پر ان کو دینی حمیت و غیرت اور مغرب سے عدم مرعوبیت کی دعوت دیتے تھے، ان کی تقریروں اور تحریروں کے متعدد مجموعے شایع ہوچکے ہیں۔ اﷲ تعالیٰ ان کی خدمات کو قبول کرے اور مغفرت فرمائے۔ آمین!! (’’ع ۔ع‘‘، اگست ۱۹۹۹ء)

کاروباری تشہیر اور اخلاقیات: سیرت طیبہ کی روشنی میں

The History revealed the fact that merchants played a very prominent and vital role in spreading the religion of Islam. Prophet Muhammad (PBUH) remained one of the popular merchants of his time in the Arabian Peninsula. Similarly, the same profession was also adopted by the Prophet’s companions and they propagated the message of Islam almost in every corner of the world being a core mission of their lives. The number of Islamic countries on the world’s map witness their efforts in this regard. There are numerous sayings of the Prophet (PBUH) in which he praised the honest and truthful traders and cautioned with serious wrath for the otherwise. Keeping in view the contemporary scenario of corporate world, one can easily observe the lacking of professional and ethical values among the merchants more specifically concerned with commercialization and marketing. In fact, marketing is one of the sound sources to promote business but some companies try to exploit their consumers via fake commercialization and false advertising. Pakistan, being an Islamic country, has to devise such principles and regulations regarding commercial advertising which are in full conformity with Shariah standards that will definitely, secure the rights of all stakeholders along with fine attraction for foreign investment to put the economy on growing direction. The current paper deals with the morals and values of advertising in the light of Prophetic teaching.

Genetic Transformation and Expression Analysis of Cold Tolerant Gene in Tomato Solanum Lycopersicum Mill.

Chilling stress severely reduces the productivity of tomato as it is a cold sensitive plant. CBF3/DREB1A plays a key role in generating cold tolerance in tomato by regulating the response of multiple genes under chilling stress. In this study, cold tolerant gene (DREB1A) driven by Lip9 promoter, was transformed in three tomato genotypes (Rio Grande, Moneymaker and Roma) through Agrobacterium tumefaciens, employing tissue culture dependent and tissue culture independent transformation strategies. For tissue culture dependent transformation strategy, the effects of various PGRs (IAA, NAA, ZEA, Kin, BAP and GA3) and two ethylene inhibitors (AgNO3 and CoCl2) were investigated on callus induction and in vitro shoot regeneration. The maximum callus induction frequency (67.48%) was recorded on MS basal media enriched with 2.0 mg/l IAA, 2.5 mg/l BAP in cv. Rio Grande followed by Roma (62%) and Moneymaker (58.23%). Supplementation of AgNO3 (10-15 mg/l) in MS basal media along with PGRs (2.0 mg/l IAA, 2.5 mg/l BAP) significantly yielded the highest callus induction frequency (91.83%) in cv. Rio Grande, followed by Moneymaker (82.66%) and Roma (88.33%). Similarly, in vitro shoot regeneration frequency on MS media fortified with 0.1 mg/l IAA, 1.0 mg/l ZEA and 2.0 mg/l BAP significantly enhanced with the addition of 8-10 mg/l AgNO3 in all the cultivars i.e. in cv. Rio Grande (96.65%) followed by Roma (92.66%) and Moneymaker (90%). Likewise, the highest callogenesis (75.65%) was recorded in cv. Rio Grande on callus induction medium (CIM) supplemented with CoCl2 (5.5 mg/l), IAA (2.0 mg/l) and BAP (2.5 mg/l) followed by cv. Roma whose best callus induction (73.66%) was obtained on CIM supplemented with CoCl2 (4.5 mg/l), IAA (1.0 mg/l) and BAP (2.5 mg/l). In case of cv. Moneymaker the best callogenesis (68%) was secured on CIM having CoCl2 (3.5 mg/l), NAA (2.0 mg/l) and BAP (2.0 mg/l). The xiv highest in vitro shoot regeneration (85%, 81% and 78%) was recorded in Rio Grande, Moneymaker and Roma, respectively on shoot induction media supplemented with CoCl2 (4.25 – 5.0 mg/l). During this study, various concentrations of sucrose and sorbitol were scrutinized on in vitro shoot culture. The highest in vitro shoot regeneration frequency (100, 97.69 and 99%) was recorded in Rio Grande, Moneymaker and Roma with accumulative effect of sucrose and sorbitol (30: 30 g/l). Subsequently, transformation experiments were conducted by optimizing various factors both for tissue culture based and in planta techniques. For tissue culture based method of transformation; fifteen days old in vitro seedlings, forty-eight hours pre-culture period, bacterial density (OD600 nm = 0.2), three minutes infection period, 60 μM acetosyringone, forty-eight hours co-cultivation period, pH 5.6 of co-cultivation media, six days pre-selection duration, cefotaxime (500 mg/l) and hygromycin (35 mg/l) as lethal dose were found optimum. For in planta technique of transformation, various factors such as growing medium; soil: vermiculite (1: 1), optical density (OD600 nm = 1.0) and incubation period (20 min) were found optimum for efficient transformation efficiency. Polymerase chain reaction, Multiplex polymerase chain reaction, Southern blotting and Reverse transcriptase PCR confirmed the presence, integration and expression of DREB1A in T0 - T2 transgenic lines. Physiological and biochemical analyses of T2 transgenic plants depicted that after various chilling stresses; stomatal conductance, transpiration rate, CO2 concentration rate, photosynthetic rate, relative water contents, proline contents, total soluble sugar contents, chlorophyll contents, carotenoid contents and ascorbic acid contents of transgenic lines were significantly higher than those of NT plants. These findings clearly indicate that transgenic tomato plants over-expressing Arabidopsis CBF3 gene enhanced protection and provided cold tolerance under controlled conditions in transgenic containment.
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