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الحاج منظور علی لکھنوی

الحاج منظور علی لکھنوی مرحوم
دارالمصنفین میں الحاج منظور علی لکھنوی کی رحلت کی خبر بڑے افسوس کے ساتھ سنی گئی، ۲۸؍ رمضان المبارک کو وہ اپنے خالق حقیقی کے جوار رحمت میں پہنچ گئے، اناﷲ وانا الیہ راجعون۔
وہ زندگی میں پاکیزہ اطوار رہے، آخری وقت بھی اس پاکیزگی کی شہادت کے لیے قدرت نے مقرر کردیا، وہ سیاسی رہنما تھے اور نہ عالم و فاضل لیکن ان کی زندگی قطرے سے گہر ہونے کی کہانی ہے، کلکتہ کے ہوٹل بزنس سے تعلق تھا، ان کا رائل انڈین ہوٹل کلکتہ کے مشہور ترین ہوٹلوں میں ہے، تمول کی آغوش میں آنکھیں کھولیں، عام امیر زادوں کا رنگ ڈھنگ ہونا قدرتی تھا، ایک وقت تھا کہ قیمتی پتھروں اور بیش قیمت خوشبوؤں کو جمع کرنے کا شوق تھا لیکن فطرت کی سلامتی اور سرشت کی پاک طینتی نے ایک دن ان کی زندگی کا رخ بدل دیا، ثروت کو اﷲ کی دی ہوئی نعمت سمجھنے اور برتنے کی توفیق، باندازۂ ہمت ملی اور زندگی قابل رشک ہوگئی، دارالعلوم ندوۃالعلما کی مجلس انتظامیہ، مسلم پرسنل لابورڈ، ملی کونسل، امارت شرعیہ جیسے باوقار اداروں کی رکنیت اور سب سے بڑھ کر حجاج کرام کی پیہم خدمت نے ان کو اپنے طبقہ میں امتیاز کا شرف بخشا اور اس سے زیادہ کلکتہ میں ان کی وہ رفاہی خدمات ہیں جن کا علم بہ جزاﷲ اور ان کے انتہائی قریبی لوگوں کے اور کسی کو نہیں معلوم، کمال کے شخص تھے مولانا عبدالماجد دریا بادی کو کبھی نہیں دیکھا لیکن ان کی تحریروں کے ایسے شیدائی ہوئے کہ ان کی کتابوں کی خوبصورت ترین اور نہایت دیدہ زیب طباعت و اشاعت کے لیے بغیر کسی تاجرانہ فائدے کے، اپنے مال کا بے دریغ استعمال کیا، ادارہ انشائے ماجداسی نیت سے قائم کیا جس نے خطبات ماجد سے مکتوبات ماجدی تک مولانا...

MODEL PEMBELAJARAN BAHASA ARAB YANG TERFOKUS PADA SISWA (STUDENT-CENTERED LEARNING/SCL)

This paper would like to descripte the characteristics of learning that focused on the learners, the benefits, the weaknesses and the steps of its use to achieve the goal of learning Arabic. Learning model is an important part which is understood by every Arabic teacher to be able to present the condition of learning that is always fun for learners. An effective model for learning in Arabic is the learning model that concentrated to student. The use of this learning model is believed to be able to improve the four competencies of Arabic learners, namely the ability to speak, write, read and hear. For this reason, an Arabic teacher must understand the various variants of an effective learning model which centered on the learning potential of students in order to present the materials in an enjoyable. Therefore, it is important for any Arabic teacher to change the teacher-centered paradigm which is conventional teaching towards a modern learning paradigm which centered to the creative ability of students during the Arab learning proccess. The use of learning models which focused to learners in Arabic learning should be supported by teacher innovation that can empower learners to be active during learning. In addition, the teacher seeks to construct the latest Arabic material by utilizing the technology media relevant to the progress of the global education world, especially in learning Arabic as the language of the Islamic world and religious language.

Dna Profiling of Coprophilous Fungus Sordaria Fimicola by Pcr Based Molecular Markers

The present study is restricted to the isolation, morphological and molecular characterization of Sordaria fimicola by applying PCR based molecular markers coupled with high resolution melt analysis to obtain adequate data for measuring genetic variations. All the collected samples isolated from different herbivore dung samples were plated; incubated and S. fimicola colonies were identified on solid PDA media. A total of 61 fungal isolates of S. fimicola were isolated by moist chamber and single plate isolation techniques. The effect of different physical parameters such as pH (4.5-8), temperature (20-45 ºC), carbon (cellulose, glucose and maltose), nitrogen sources (sodium nitrate, ammonium sulphate and casein hydrolysate) and UV light on the mycelial and perithecial growth was also determined on different strains of S. fimicola. Present attempt was made to gauge molecular differences on Sordaria fimicola‟s genome present on site of Evolution Canyon. All strains and isolates were authenticated by targeting internal transcribed spacer and hypervariable regions (ITS1, ITS2, full ITS, V3, V4 and V9). No sequence variations were observed in case of ITS and V9 regions while two polymorphic sites on position 212 A (C); 213 G (A) were found in V3 and V4 region in strains isolated from the south slope of Evolution Canyon. The PCR-based technique of randomly amplified polymorphic DNA (RAPD) was used to fingerprint and assess the genetic relatedness among eight isolates of the fungus S. fimicola isolated from various areas of Lahore, Pakistan. Each DNA sample was amplified with each of eight RAPD primers during the initial screening and the products were resolved on 1.5 % agarose gel, stained with ethidium bromide and snapped under gel documentation system. ii Four primers failed to show any amplification but the remaining four (50 %) primers generated a total of 22 bands (5.5 bands per primer) among the eight isolates of S. fimicola. Of these 22 resolved bands, 13 (3.5 per primer) were polymorphic and the remaining 9, bands were common (monomorphic) among these isolates. The least efficient primer was R8 (1.79 %), while the most efficient one was R3 (8.93 %). Primer R3 had the highest PIC value (0.5) and identified all eight isolates through unique patterns of banding. Jaccard similarity coefficient ranged between 0.2-0.6. Sequence products of 485 bp and 811 bp long nucleotides were obtained from two RAPD loci (RL-7 and RL4) and converted to sequence characterized amplified region (SCAR400 and SCAR500) markers for S. fimicola. Another aim of the experiment was to look for natural genetic variation in different natural strains of Sordaria fimicola isolated from natural and stressed ecological conditions of Evolution Canyon. It was challenging because there was no sequence information at the time, and I was required to design a set of PCR primers that would reliably amplify in S. fimicola and it was hoped that the amplified products were polymorphic. Hence the decision was made to target randomly selected genes and regions containing short sequence repeats, putting primers in the exonic parts to amplify the intron(s) they flank and used S. macrospora sequence information to strategically place the "exon-primed, intron-crossing" primers to determine nucleotide variations in fifty strains of Sordaria fimicola by sequencing PCR amplicons. As compared to the strains isolated from the north slope, strains isolated from the south slope exhibited point mutations on various positions and enrichment of short sequence repeats was observed more in strains isolated from the south slope of EC. Frequency clock proteins are significant factor of circadian clocks which regulate gene expression that react to various environmental conditions in many organisms and are iii principally involved in rhythmic movements displayed by numerous filamentous fungi. Mating type a-1 proteins, encoded by mat-a-1 genes, control the sexual compatibility and vegetative incompatibility with A mating types in many ascomycetes. In this study, I have also explored the phosphorylation and glycosylation status of frequency clock and mating type a1 proteins on serine/threonine/tyrosine residues using NetPhos3.1 and YinOYang 2.1 server.
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