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امجدؔ حیدرآبادی

امجدؔ حیدرآبادی
افسوس ہے کہ پچھلے دنوں حضرت امجد حیدرآبادی کا۸۳ سال کی عمر میں انتقال ہوگیا۔مرحوم بڑے قادر الکلام اورنغز گوشاعر تھے۔نثراورنظم دونوں میں یدِ طولیٰ رکھتے تھے۔لیکن رباعی گوئی اُن کا خاص فن تھا۔اس میدان میں وہ اپنا کوئی حریف نہیں رکھتے تھے،اس اعتبار سے و ہ درحقیقت اردو زبان کے سرمد تھے۔ چنانچہ مولانا گرامی نے بجاطورپر کہاتھا:
امجد بہ رباعی ست فردا مجد
کلکِ امجد کلیدِ گنجِ سَرمد
گفتم کہ بودجوابِ سرمدؔ امروز
روحِ سَرمد بگفت امجد امجد
 بلند پایہ شاعرادیب اورمصنف ہونے کے علاوہ بڑے صاحب ِدل،صاحبِ معرفت،خوددار،غیور اورپابندِ وضع بزرگ تھے۔زندگی بالکل درویشانہ ا ورقلندانہ تھی۔عسرت وافلاس سے ہمیشہ سابقہ رہا مگراربابِ ثروت ووجاہت کے ساتھ نیازمندی کاتعلق رکھ کراپنے فن اورکمال کی توہین کبھی گوارا نہیں کی۔سیلاب رودِموسی کے واقعہ کے بعد جس میں اُن کے دیکھتے ہی دیکھتے بوڑھی ماں اورجوان بیوی بہہ گئیں اورغرق ہوگئیں تھیں وہ سوزوگداز مجسم اورپیکر عبرت ہوکررہ گئے تھے لیکن کیا مجال کہ تسلیم و رضا کی پیشانی پرکوئی بل بھی پڑا۔مولانا سید مناظر احسن گیلانی اورمرحوم میں بڑے مخلصانہ تعلقات تھے اوردونوں ایک دوسرے کی دل سے قدرومنزلت کرتے تھے۔مرحوم سے راقم الحروف کی پہلی ملاقات ۱۹۴۴ء میں حیدرآباد میں مولانا کے مکان پرہی ہوئی تھی۔یہ ملاقات اگرچہ سرسری تھی، لیکن مرحوم کے حافظہ کاکمال یہ تھا کہ ۱۹۵۸ء میں جب سفرحیدرآباد کے سلسلہ میں موصوف کے مکان پرحاضر ہوا تو اگرچہ ضعیفی اورحافظہ اورمسلسل علالت کی وجہ سے بہت کمزور ہورہے تھے اوربینائی بھی بہت کمزور ہوچکی تھی، مگر باایں ہمہ میری آواز سنتے ہی فوراً زنانخانہ سے مردانہ میں آگئے۔ بڑی شفقت ومحبت سے خاطر تواضع کی، دیر تک باتیں کرتے اوربرہان و ندوۃ المصنفین کی خدمات کاتذکرہ کرتے رہے۔چلنے لگا تواپنی تمام کتابوں کاسیٹ عطافرمایا،میں نے اُن کاہدیہ پیش کیا تومیرے سخت اصرار کے بعد بادل نخواستہ قبول فرمایا۔...

Can the Subalterns Sketch? A Critical Semiotic Analysis of the Novel‘ Munnu: A Boy from Kashmir’

This paper is formulated under the conceptual framework of Gayatri spivak’s theory of Can Subaltern Speak? And explores the potential permeability of visual resources as a form of discourse through which subalterns found opportunity to counter media hegemony and make their voices heard. In order to analyze the selected graphic novel Munnu: A Boy From Kashmir (2015) the present qualitative study applies Grunter Kress and Theo Van Leeuwen’s model of visual design (2010) that connects the representational meaning to the interactive one. The analyst reflected upon how the interplay of visuals images and words together displayed the theme of Kashmir’s subjugation as well as how this genre has proved supportive to author to counter the said hegemony. Hence, the study found consent, political domination and media control as the broad elements that can be seen in the novel and also the study concludes that counter hegemony is possible through such literary genres, as the novel’s narrator has communicated those aspects of hegemonic situation in Kashmir to a large audience through literary discourse of graphic novel genre that are chiefly absent from mainstream media’s treatments of the Kashmir’s conflict. ______

In - Vitro Modulation of Human Glioblastoma Cells U87 by N- 2 Hydroxy Pheny Acetamide

Glioblastoma multiforme (GBM) is the most common and malignant primary brain tumor. It accounts for more than 60% of all brain tumors in adults. Treatment options available for malignant gliomas include gross total resection, radiation therapy and chemotherapeutics, e.g., temozolomide, carmustin or carboplatin. In spite of the variety of modern therapies against GBM, it is still a deadly disease with extremely poor prognosis. Patients usually have a median survival of approximately 14-15 months. The development of new therapeutic strategies for the management of gliomas is therefore crucial. The present study is designed to analyze the therapeutic potentials of anti-inflammatory compound N-(2-hydroxyphenyl) acetamide (NA-2) and anti-hypertensive drug Verapamil (VP) in the treatment of GBM as well as their combine effect with standard drug Temozolomide (TMZ) on U87 human glioblastoma cells. MTT assay was used to determine the growth inhibitory effect of NA-2, VP and TMZ. It was observed that these three drugs inhibited growth of U87 in dose dependent manner. The IC50 doses of NA-2, VP and TMZ were found to be 1.7 mM, 0.45 mM and 0.134 mM respectively. To find out whetherapoptosis is involved in growth inhibition, cells were treated with IC50 doses of these drugs and the effect was observed on morphology, chromatin condensation and DNA fragmentation using phase contrast microscopy, DAPI staining and TUNEL assay respectively. In all three treatment groups cells showed apoptotic morphology, chromatin condensation and DNA fragmentation which are hallmarks of apoptosis. Apoptosis is a process of programmed cell death regulated by pro-apoptotic (Bax) and antiapoptotic genes (Bcl-2). Deregulation of these genes is found to be linked to gliomagenesis. Hence an increase in Bax/Bcl-2 ratio favors the process of apoptosis. Caspase-3 is also an important protein that acts downstream to Bax/Bcl-2 and involved in the execution of apoptosis. Keeping in mind the importance of these apoptotic markers we also studied the effect of NA-2, VP and TMZ on these markers to find out their possible mechanism of action. Cells were treated with IC50 doses of these drugs for 24 hrs and RT-PCR was performed to see the mRNA expression of these markers in vehicle control and the three treatment groups. It was observed that after 24 hrs of treatment both NA-2 and VP downregulated Bcl-2 expression while TMZ has not shown any significant effect on the expression of Bcl-2 as compared to vehicle control. Baxand caspase-3 expression was found to be significantly up-regulated in NA-2, TMZ and VP as compared to control. Beside these proapoptotic and anti-apoptotic proteins we also studied the effect of these drugs on the expression of two other cellular markers that are involved in growth and proliferation of glioma i.e. HIF-1α and VEGF. Both NA-2 and VP inhibited the expression of VEGF and HIF-1α which is a transcriptional regulator of VEGF. While TMZ has not shown any significant effect on these markers. Next, the effect of NA-2 and VP was studied on growth inhibition after combining them with TMZ in various different concentrations. Coefficient of drug interaction (CDI) was also calculated. It was found that combination of 0.33 mMNA-2 with 0.1 mM of TMZ and 0.025 mM of VP with 0.1 mM of TMZ produced synergistic effect on growth inhibition with CDI value < 1. Combination doses that produced synergistic growth inhibitory activity were used for further studies. TUNEL assay was used to detect apoptosis in combination treatment group (TMZ + NA-2 and VP + TMZ) and individual drug treatment groups i.e NA-2 (0.33 mM), TMZ (0.1 mM) and VP (0.025) using doses that produced synergistic effect. Results of TUNEL assay revealed that even low doses (mentioned above) of NA-2, TMZ and VP induced apoptosis and this apoptotic effect was more pronounced in combination treatment groups (TMZ + NA-2 and VP + TMZ) as compared to control and individual drug treatment groups. To determine the possible mechanism of action involved in synergism we studied the effect of NA-2, TMZ and VP individually and in combination (TMZ + NA-2 and VP + TMZ) on the same molecular markers that we studied at IC50doses of these drugs. Expression was analyzed at both mRNA and protein level using RT-PCR and Immunocytochemistry. NA-2, TMZ and VP (non significant mRNA) up-regulated the expression of Bax at both mRNA and protein level andthe expression was more pronounced in combination treatment group TMZ + NA-2 as compared to individual treatment of NA-2 and TMZ. In case of VP + TMZ no further increase in expression was observed as compared to TMZ only. In contrast to BAX, Bcl-2 was found to be down regulated after treatment with NA-2 and VP as compared to control while TMZ had no significant effect on the expression of Bcl-2. Moreover No further significant down-regulation of Bcl-2 was observed at protein level when NA-2 and VP alone treatment groups were compared with TMZ + NA-2 and VP + TMZ respectively. Increase in Bax expression by NA-2, TMZ and VP and down-regulation of Bcl-2 by NA-2 and VP only leads to dramatic increase in Bax/Bcl-2 ratio and shifted the equilibrium of cells towards apoptosis. Apoptosis was further confirmed by analyzing active Caspase-3 expression. NA-2, TMZ and VP treatment also increased active caspase-3 expression and the expression was highest in combination treatment groups where Bax/Bcl-2 ratio and apoptosis was also highest as compared to control and individual treatment of the drugs. Here we concluded that the synergistic growth inhibition that was observed in combination treatment group may in part be related to increase in apoptosis. The expression of HIF-1α and VEGF was alsoanalyzed in combination treatment and found similar results that were observed at IC50 doses. Both NA-2 and VP inhibited the expression of HIF-1α and VEGF while TMZ had no effect on the expression of both these marker. No further significant down-regulation was observed when NA-2 + TMZ and VP + TMZ were compared with NA-2 and VP alone treatment group respectively. In contrast to NA-2 and VP, TMZ did not have any significant effect on the expression of HIF-1α and VEGF, it is possible that increase in efficacy and growth inhibitory activity of TMZ in combination treatment group might also be related to the NA-2 and VP mediated down regulation of HIF- 1α and VEGF as both the markers have role in growth and proliferation also. Based on our observations, we conclude that NA-2, VP and TMZ can inhibit the growth of U87 glioblastoma cells by inducing apoptosis. NA-2 and VP may also inhibit proliferation by down-regulating HIF-1α and VEGF expression. Synergistic growth inhibitory activity of NA-2 and VP with TMZ may in part be related to their apoptotic and anti-proliferative activity. In short NA-2 and VP both have growthinhibitory activity alone which was further refined in combination with TMZ and they can be exploited for therapeutic purposes.
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