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۵-اين

۵-اين

"يَقُوْلُ الْاِنْسَانُ يَوْمَىِٕذٍ اَيْنَ الْمَفَرُّ"[[1]]

"اُس وقت یہی انسان کہے گاکہاں بھاگ کر جاؤں؟"۔



[[1]]     القرآن ، ۷۵: ۱۰۔

Diversity Analysis of Rotifers from Temporary Spring Pools of Jallo Park, Lahore, Pakistan

Temporary pools are relatively small water bodies with limited resources and are seasonally available. These freshwater bodies are rich source of microbial and planktonic lifeObjective: This survey was conducted to find out the presence of rotifers in temporary pools formed inJallo Park established in 1978 and explore their density and diversityMethods: Sample collection was done from ten pools with the help of a planktonic mesh. The counting was performed with a Sedgwick- Rafter chamber. Diversity indices like Shannon Weaver index etc. Were applied Results: In present study 27 rotifer species belonging to 14 genera were identified. The highest diversity and density was shown by Brachionus and Lecane. There was even distribution in each pool. Results of ANOVA showed statistically significant difference in population density and diversityConclusions: This study has presented a guideline to find out rotifer diversity which could be present in any water reservoir like temporary pool.

Isolation and Characterization of Cadmium Metallothionein Gene from Local Ciliates

First cadmium metallothionein like gene PMCd1 of a ciliate, Paramecium sp., isolated from indus- trial wastewater has been cloned and sequenced. PMCd1 is an intronless gene, encoding 612 nucleotides, with TAA coding for glutamine. The coding region of PMCd1 com- prises 203 amino acids, including 37 cysteine residues with a conserved structural pattern in the form of recurring structural motifs, arranged in 17 x-cys-x-y-cys-x, 1 x-cys- cys-x and x-cys-x contexts. Both, the deduced amino acids and nucleotide sequence differ, not only from other animal metallothioneins (MTs), but also from the previously characterized Tetrahymena Cu and Cd-MTs. The translated protein of PMCd1 contains conserved cysteine residues, peculiar characteristic of stress inducible metallothionein genes of ciliates and other groups of organisms.
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