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حرفے چند

ارضِ اقبال آفاقیت کے آئینے میں‘‘ڈاکٹر نصیر احمداسد کے مختلف تحقیقی و تنقیدی نوعیت کے مضامین پر مشتمل کتاب ہے۔بیشتر مضامین مختلف تحقیقی و تنقیدی رسائل کی زینت بن چکے ہیں۔ اُنھوں نے جن جن شخصیات پر قلم اٹھایا ہے،ان کے بارے میں معلومات کا ایک ذخیرہ جمع کر دیا ہے۔جہاں جہاں ضرورت محسوس کی ہے اپنی ناقدانہ رائے کا اظہار بھی بڑی خوب صورتی سے کیا ہے۔
    مَیں نے ڈاکٹر نصیر احمد  اسدکے مزاج میں ایک خاص بات محسوس کی ہے کہ وہ اپنے کام سے نہایت مخلص ہیں اور اپنی دُھن میں علمی خدمات میں ہمہ وقت مصروف  نظر آتے ہیں۔ان کے اسلوب کی سادگی اور روانی بات کو بوجھل نہیں ہونے دیتی۔ ان مضامین کے مطالعہ سے قاری نہ صرف مختلف شخصیات سے متعارف ہوتا ہے بلکہ ان کی علمی خدمات سے بھی ایک حد تک مستفید ہوتا ہے۔
    ’’مولوی فیروز الدین ڈسکوی‘‘ اور’’مولوی ابراہیم میر سیالکوٹی‘‘ والے مضامین میں مذکورہ شخصیات سے متعلق عمدہ معلومات جمع کی گئی ہیں۔ان شخصیات کے تعارف کے ساتھ ساتھ ان کی علمی خدمات پر بھی روشنی ڈالی گئی ہے۔اسی طرح ’’سیالکوٹ میں اردو شاعری کا ارتقا‘‘ اور’’ریاض حسین چودھری ایک نعت گو شاعر‘‘ میں ناقدانہ انداز نظر سے کام لیا گیا ہے۔دیگر مضامین بھی اپنی نوعیت کے خوب صورت مضامین ہیں۔الغرض مذکورہ کتاب ان کی تحقیقی و تنقیدی بصیرت کی آئینہ دار ہے۔
ڈاکٹر محمد افضل صفی
صدر شعبہ اردو گورنمنٹ گریجوایٹ کالج ،کروڑ لعل عیسن، ضلع لیہ

The Impact of Nicotine and Social Drinking Behavior and Brain Function

Nicotine acts on nearly every Physiological System of the human body. The effects of nicotine on the Peripheral nervous system have been extensively studied and are now quite well understood. The effects of nicotine on the central nervous system are more complex, and our understanding of these effects is limited. The Pharmacokinetic and Pharmacodynamics of nicotine, with an emphasis on the Psychopharmacological basis of nicotine dependence. In South America, seeking for psychoactive effects of nicotine might be as old as the origin of horticulture, beginning some eight thousand years ago present Ritual tobacco was used in shamanism aimed to achieve acute nicotine intoxication, which induced in the shamans’ catatonic states representing symbolic death. The effect of large doses of nicotine on the autonomic and central nervous system gave the impression of a gradual death of the shaman, who then returned miraculously to life (Wilbert 1987). Regular, moderate use of nicotine alone or in combination is a well-known, widely established and loved practice of men and women both. Despite, health consequences of these pharmacological agents, people continue, quite persistently, to consume these substances and afford much value to the pleasure of a regular intake.

Effect of 5 Deletions of Efficiency and Specificity of Osrglp2 Promoter

Germin like proteins (GLPs) are member of a large gene family and ubiquitously expressed as plant proteins. The exact mode of action and role in metabolism is not well understood. It is believed that these putative stress proteins are expressed at various developmental stages in response to abiotic and biotic stresses. The present study was designed to clone full length and 5'' abbreviated Oryza sativa Root expressed GLP2 (OsRGLP2) promoter, its genetic transformation into Arabidopsis and characterization. Cloning of full length and 5'' abbreviated OsRGLP2 promoters was carried out by employing GATEWAY™ Technology. Amplification was performed by specific primers designed on the promoter region. Recombinant entry clones were created by using pENTR/D-TOPO® cloning kit. Expression vectors were prepared by employing LR recombination reaction between recombinant entry clones and promoter less destination vector pHGWFS7. Confirmation was carried out by PCR and sequencing. Recombinant expression vectors were named as pNSS-F1 (Full length promoter) and pNSS-F2, pNSS-F3 and pNSS-F4 (5'' deleted promoters). Plant transformation into Arabidopsis was carried out by Agrobacterium mediated floral dip method. T0 and T1 lines were established and transgenic plants were analyzed by molecular and physiological approaches. T1 transgenic lines for each promoter constructs were selected through GUS assay and tested for wound, salt and temperature stresses. Real-time PCR was performed by using two sets of primers, Actin (Housekeeping gene) and GFP (Gene of Interest). Real-time PCR analysis was done by employing 2-ΔΔCt method in which data was normalized by software inbuilt in the real-time PCR system by taking calibrator or untreated sample value xxi as 1. The graphical data shows the times fold increase or decrease in the expression with comparison with untreated control samples. Expression analysis revealed that OsRGLP2 promoter is efficient and specific to wound, salt and temperature stresses. When comparison was done between full length and 5'' deleted promoters, the promoter named pNSS-F3 of 565 bp along with other two larger promoter pNSS-F1 and pNSS-F2 of sizes 1063 bp and 776 bp respectively were responding to all stresses applied during this study. However, when a further deletion was made and shortest promoter pNSS-F4 was created which comprises of 283 bp size, unable to respond against stresses applied. So it can be concluded from present study that during 5'' deletion of full length OsRGLP2 promoter (creation of pNSS-F4), a critical region between P-565 and P-283 on promoter fragment was deleted which contain promoter elements necessary to respond against certain stresses like wound stress, salt stress and temperature stress. While during other deletions (in case of pNSS-F2 and pNSS-F3) in which when the critical part was intact, the promoter responded to applied stresses. It can be further concluded that pNSS-F3 is a good replacement for full length promoter. Due to smaller size of pNSS-F3 promoter (565 bp) and it‟s efficient and specific response against abiotic stresses it can be fused with other promoter and used in combination against certain abiotic or biotic stresses.
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