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اساتذہ

اساتذہ

 مفتی اعظم ہند، مفتی عزیز الرحمن عثمانی،غلام بشیر احمد عثمانی اور مولانا اعزاز علیؒ شامل ہیں اور دورہ حدیث میں آپکے اساتذہ انور شاہ کشمیری غلام رسول ہزاروی تھے اور مولانا اشرف علی تھانویؒ سے بھی حدیث کی تعلیم حاصل کی۔

فتوی کی ذمہ داریاں

افتاء کا منصب علمی سلسلوں میں سب سے مشکل سمجھا جاتا ہے فقہ کے لاکھوں ملتے جلتے مسائل کا تھوڑے تھوڑے فرق سے حکم بدل جاتا ہے۔ بہت سے احکام اور حالات کے تغیر سے بھی بدلتے ہیں دار العلوم دیو بند میں تدریس کا جب آغاز کیا تو اس وقت دارالعلوم کے صدر مفتی حضرت مولانا عزیز الرحمن عثمانی ؒ تھے ۱۳۴۴ھ میں مفتی اعظم ہند جب دارالعلوم سے مستعفی ہوگئے تو مفتی شفیع ؒکو منصب افتاء کی پیش کش ہوئی جو انہوں نے مولانا اشرف علی تھانویؒ کے مشورے سے قبول کرلی۔اور ۱۳۵۰ھ کو دارالعلوم دیو بند کی مجلس شوریٰ نے آپ کو منصب افتاء پر بحیثیت صدر مفتی فائز کردیا۔

فتوی سے تدریس کی طرف منتقلی

 بزرگوں کے حکم پر فتوی کی ذمہ داری کو قبول تو فرمالیا مگر بعد میں تدریس میں واپس چلے جانے کی اجازت چاہی لیکن اجازت نہ ملی آپ کے دوبارہ اصرار پر ۱۳۵۴ھ میں دارالعلوم کی مجلس شوری نے یہ مشکل فیصلہ بھی کردیا کہ فتویٰ سے تدریس کی طرف منتقل کردیا جائے۔

سیاسیات میں فکری و عملی حصہ

طبعاً ہنگاموں اور جلوسوں سے الگ رہنا پسند کرتے تھے لیکن جب بھی دین اسلام اور مسلمانوں کی کسی اہم دینی ضرورت نے سیاست میں حصہ لینے کا تقاضا کیا تو آپ اس میں شریک ہوئے۔

 پہلی جنگ عظیم کے اواخر میں جب مجاہدین بلقاں ہر طرف سے کفر...

THE EFFECTS OF A PRAGMATIC SET OF INTERVENTIONS ON THE SHOULDER RANGE OF MOTION IN MALES AND FEMALES WITH SHOULDER PAIN: A CLINICAL TRIAL

Background and Aim: The effects of novel set of interventions are known but their effects with respect to gender are not known. This study aim to determine the effects of novel set of interventions on shoulder range of motion in males and females with shoulder pathology. Methodology: This study was of quasive experimental design. Thirty subjects of mean age (±SD) of 43. 23±10 years with shoulder pathology and restricted ROM were recruited. The major criteria for recruitment were 18-60 years of age. The general contraindications of manual therapy were the exclusion criteria. Shoulder functional movement and range of motion were the outcome measures.  Results: The mean % (SD) change for RUBB was 15.04±11.57for males and  14.49±10.44 for males. The change for RDBN was also significant (<0.00) from baseline and the % change in mean was 14.93±11.0 for males and 12.60±9.06 for females. The changes were well above the highly clinical meaningful difference (>0.8). It is further observed that the differences in gender were non-significant (P>0.05). Conclusion: The pragmatic set of interventions affect both the genders equally and improve shoulder range of motion and functional movements.  However, the results must be interpreted cautiously because of the inadequate sample size.

Cloning and Expression of Cdna Encoding Bioactive Venom Proteins from the Mealbug Parasitoid Aenasius Arizonesis Girault =Aenasius Bambawalei Hayat Hymenoptera, Encyrtidae

The Endoparasitoid Aenasius bambawalei Hayat (Hymenoptera, Encyrtidae) has been synonymized with Aenasius arizonensis (Girault) (Hymenoptera, Encyrtidae) in 2014. It is a newly emerged parasitoid of mealybug Phenacoccus solenopsis Tinsley (Hemiptera, Pseudococcidae) and it has been found an efficient insect control tool. There is little information available on parasitoid origin factors responsible for modulation of mealybug physiology. Parasitoid’s venom contains biologically active proteins that have potential applications in pest management, some of them also have medicinal importance but venom components of A. arizonensis have not been studied yet. Venom glands tissues of A. tarizonensis were used for transcriptomic study and transcriptomic database was developed by using high throughput RNA sequencing approaches using Illumina Technology. The transcriptomic data of A. arizonensis venom glands was analysed by utilizing high throughput sequencing Illumina technology and de novo assemblies were constructed, containing 30,267,259 sequences reads which yielded 30,154,362 contigs and 8,507 unigenes which had significant BLAST homologies n the NR database. The database sequences showed homology to 2666 Nasonia vitripennis genes, 2065 Copidosoma floridanum genes, 1660 Ceratosolen solmsi genes, 1598 Trichogramma pretiosum genes and 1192 Cerapachys biroi genes. Further analysis was performed by selecting some genes encoding venom proteins which are potentially involved in the disruption of host immune system, developemental arrest and host paralysis. Sequenced mRNAs predicted to encode full length ORFs of Calreticulin, Arginine kinase and serine protease precursor proteins were identified, and tissue specific expression of these putative venom proteins was performed by RT-PCR which reveals that venom genes were not only exclusively expressed in venom tissues but also conserved in all carcasses of the parasitoid species. Application of crude venom and expressed proteins on cultured cell lines showed valuable results for understanding that there are paralytic factors in parasitoid venom which cause cell death. Whereas in functional analysis of microinjections, venom treated with heat and proteinase showed non-significant mortality which suggests that bioactive components of the crude venom were proteins which lost their biological activities upon heat treatments. Additionally, results also demonstrated that transcriptome de novo assembly allows useful venom gene expression analysis in species lacking genome sequence database which ultimately provide useful information for devising control tools for insect pest. This work also contributes to the understanding of the molecular and physiological bases of host parasitoid interaction in insects that may provide an unexplored resource for diverse biotechnological application and useful information for entomologists seeking to devise sustainable control strategies for cotton mealybug.
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