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عظیم ذات ہے اُس کی، بزرگ و برتر ہے (حمد)

حمدباری تعالیٰ

عظیم ذات ہے اُس کی بزرگ و برتر ہے
نہ کوئی ثانی ہی اُس کا نہ کوئی ہمسر ہے

جو بحر اُس کا ہے سارا تو اُس کا ہی بر ہے
جو خشک اُس کا ہے سارا تو اُس کا ہی تر ہے

وہی تو ہے جو ہے ذات و صفات میں یکتا
ہر ایک رنگ میں بے مثل وہ سراسر ہے

ہیں دو جہاں پہ عطائیں تمام تر اُس کی
وہ بے عدیل سخی ، بے مثال داور ہے

یہ اور بات کہ ہے لا مکاں مکاں اُس کا
مگر جو ہے دلِ مومن وہ اس کا ہی گھر ہے

کتب سماویہ اور انسانی فکر کا ارتقاء ایک علمی و تحقیقی جائزہ

Allah has created human being and gave him the power of intellectual understandings. To utilize and make positive use of this Allah sent Holy books for guidance. While, keeping the sequence of these books and the historical evolution of human mind, one can easily recognize the symmetrical progress in both. Human starts living in caves and through his intelligence created homes to live, tools to gain meal, and so on, till todays growth. As far as, evolution of Holy books is considered, Scriptures were revealed in different portions as human at that stage couldn’t write or store on pages or another source. Old Testament was revealed earlier. It is combination of Torah, Psalms and other books. In the context of Islam, Torah was the first book revealed on Hazrat Mosa, in which commandments were given as per the level of Bani Israel. Afterwards, Psalms, containing poetic way inspires Bani Israel after the Torah and the Bible Last Holy book in the generation of Israel, comprises of observational and experiential way, as required in that era. Holy Quran, compiled and final version of all the past version, is summary and elemental book. In which, descriptive, observational, experiential, poetic, logical, argumentative and comprehensive technique are present. These all shows, ultimate intellectual growth of human is being address in Holy Quran. As Human is now intellectually at its peak so the teaching and techniques used are at highest level of literature, showing the step by step evolution and association of Holy books with Human being.

Molecular Detection and Charaterization of Escherichia Coli Isolated from Raw Cow Milk Samples Collected from Bahawalpur District

Milk is generally considered as highly nutritious and useful food for all age groups as well as inexpensive and widely available. However, its quality and microbial content are the features which have to be observed. The quality of milk depends on its biochemical composition and hygienic conditions during the collection of milk and its distribution. Milk itself acts as best medium for enhancing the growth and development of different types of microbes because of its composition and presence of water in large quantity. Milk sold in Bahawalpur district was suspected to be contaminated because of repeated outbreaks of gastroenteritis. In this context, the research was designed to rule out molecular detection and quantitative analysis of Escherichia coli (E. coli) in the milk samples obtained from various tehsils of Bahawalpur district. E. coli were considered as the most prevalent bacteria of milk and could be the most probable microbe causing this disease. In this research, 100 raw milk samples were gathered in a way that 10 samples each were collected from Jamalpur, Hasilpur, Khairpur, Qaimpur, Lal Sohanra, Lal Sohanra National Park, Yazman Mandi, Head Rajkan, Ahmedpur East and Uch Sharif. Then the samples of milk were cultured on different culture media for bacterial segregation. Identification of bacterial specie was done through gram?s staining and properties of bacterial culture on different selective media. Biochemical tests were also performed which include catalase and coagulase tests. Final identification was performed through PCR and resolution of PCR products by gel electrophoresis. Antibiotic sensitivity test was also performed so as to confirm the susceptibility of E. coli regarding multiple antibiotics. Out of 10 samples collected each from Jamalpur, Hasilpur, Khairpur, Qaimpur, Badar Sher, Lal Sohanra National Park, Yazman Mandi, Head Rajkan, Ahmedpur East and Uch Sharif, results had shown the presence of E.coli in 4(40%), 6(60%), 3(30%), 2(20%), 4(40%), 5(50%), 3(30%), 4(40%), 6(60%) and 5(50%) samples respectively. E. coli isolates were amplified by PCR based on 16S rRNA gene. Results of antibiotic sensitivity test revealed that E. coli isolates had shown resistance to amoxicillin (85%) and erythromycin (72%). However, they were found to be affected by azithromycin (53%), ciprofloxacin (86%), gentamicin (86%), norfloxacin (80%) and streptomycin (66%). Resistant pattern in relation to broad spectrum antibiotic (i.e., amoxycillin) points to a situation which should be considered carefully and suggests that indiscriminate use of antibiotics for precautionary or therapeutic purposes should be avoided as it could be the cause of increasing antimicrobial resistance.
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