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ڈاکٹر عادل صدیقی

ڈاکٹر عادل صدیقی(۱۹۵۴ء پ) کا اصل نام محمد شبیر صدیقی ہے۔ آپ چوہان برہانپور پسرور میں پیدا ہوئے۔ ۱۹۸۷ء میں بطور لیکچرار مرے کالج سیالکوٹ میں اپنے فرائض سنبھالے اور بعد میں پنجابی ادب میں پی ایچ ڈی کی۔ (۱۱۳۵) عادل صدیقی کی شاعری جدید رجحانات کی عکاس ہے۔ وہ اردو اور پنجابی میں بڑی مہارت سے شعر کہتے ہیں۔ اردو میں عادل صدیقی کا پہلا شعری مجموعہ ’’نوید موسم دل‘‘ شائع ہو چکا ہے۔ دوسرا شعری مجموعہ ’’غزل تم سے عبارت ہے‘‘ سنگت پبلی کیشنز لاہور نے ۲۰۰۷ء میں طبع کیا۔ حقیقت پسندی، امن و آتشی ، بھائی چارہ ،انسانی ہمدردی اور پیارو محبت عادل صدیقی کی اردو شاعری کے اہم موضوعات ہیں ۔نمونہ کلام ملاحظہ ہو:

جسم ہے پھول اگر، روح کو خوشبو سمجھو

 

بُو اڑی گل سے تو رنگت کے سوا کچھ بھی نہیں

 

â۱۱۳۶)

 

 

 

 

کاش پھر شاخِ تمنا پہ ثمر آجائے

 

;ختم ہو تیرہ شبی اور سحر آجائے

 

آبلے پا ؤں کے فریاد بلب ہیں عادل

 

اب تو خوشبو کا جہاں پیار نگر آجائے

 

â۱۱۳۷)

 

 

 

 

جب سے انسان نے رکھا ہے قدم دنیا میں

 

تب سے وابستہ ہوئے رنج و الم دنیا میں

 

پھول تقسیم کریں اپنے لیے خار چنیں

 

ایسے انساں...

Analysis of the Implementation of Patient Safety Targets at the Makassar City Regional General Hospital

The Patient Safety Goals (SKP) drive specific improvements in patient safety. These objectives highlight problematic areas of health care in a system implemented in hospitals to make patient care safer. This study aims to analyze the implementation of patient safety goals in Makassar City Hospital. This type of research is mixed methods research. The research uses a sequential explanatory strategy. The results showed that the implementation of patient safety targets based on the Hospital Patient Safety Target Standards (SNARS) at Makassar City Hospital has a good implementation of patient safety targets. The implementation of patient safety targets in terms of leadership in the Makassar City Regional General Hospital (RSUD), namely the awarding of awards has never been done, and supervision is carried out by looking at patient safety reports. In terms of human resources, training related to patient safety is still lacking and only during accreditation. Regarding policies, there are SOPs related to patient safety incidents and there is no clear sanction, only a warning. For teamwork, there is no availability of a patient safety team in the treatment room, only KMKP has a patient safety team. In addition, the implementation of patient safety goals in terms of communication, namely the existence of positive feedback given and followed up by the Patient Safety and Quality Committee (KMKP), as well as lack of socialization by KMKP, only at the time of accreditation.

Virulence Analysis of Xanthomonas Campestris Pv. Sesami and Pseudomonas Syringae Pv. Sesami the Causal Organisms of Sesame Sesamum Indicum L. Bacterial Blight

Sesame (Sesamum indicum L.) locally called as til is an important conventional oilseed crop of Pakistan. Pakistan ranks 14th among major sesame producing countries in the world. Pakistan is facing a chronic shortage in edible oil and the situation is getting serious with alarmingly explosion of population. Its indegenious production is below the utilization level and there exists wide gap between production and utilization. Sesame crop is subjected to various abiotic and biotic stresses in all stages of growth. Two prominent bacterial pathogens associated with sesame are bacterial blight caused by Xanthomonas campestris pv. sesami (Xcs) and bacterial leaf spot caused by Pseudomonas syringae pv. sesami (Psse). These pathogens are responsible for sesame production constraints during monsoon season. Despite the shortage of edible oil, no profound efforts have been made on this important oilseed crop with reference to diseases. To handle the shortage of edible oil, there was an urgent need to explore the basic information on host pathogen interaction. The present work consisted of five experiments. The first study was the standardization of mass culturing of stored Xcs and Psse isolates to enhance their virulence and confirmation of their ability to induce hypersensitive reaction. All isolates were revived on non host plant and confirmation was made on the basis of pigmentation they produced in their respective media and hypersensitive test was performed in tomato and potato plants. The second study was conducted to analyse the virulence of virulent isolates in vitro by comparing symptoms induction and bacterial multiplication in different genotypes. Plants were inoculated by pin prick method and were monitored daily for symptoms development and measurements of lesions were taken until fully symptoms induction. Bacterial populations were determined by counting bacterial colonies. Psse isolates showed necrotic lesions (chl+) surrounded by halos as well as only black necrotic lesions (chl-). Size of the lesions and bacterial population between chl+ and chl- was the same and at maximum at 7 DAI in susceptible genotypes, while tolerant showed delayed in reaction. Similar mode of lesions expansion and rate of bacterial growth between chl+ and chl- isolates of Psse indicated that the virulence factor involved in symptomatology function as pathogenicity factor and only contributed to induction of chlorotic producing symptoms for Psse. Water soaking to blight symptoms along with maximum bacterial growth in all the susceptible and moderately susceptible genotypes by Xcs was recorded at 12 DAI. The third study was conducted to confirm process of infection of these bacterial pathogens in susceptible and tolerant genotypes by light microscopy. Inoculation was done by Injection method (IM) and Bacterial suspension dip method (BSDM). Xcs colonized tracheary elements of xylem vessels through intercellular spaces of the spongy parenchyma at 7 DAI and bacterial masses were identified as dark blue infected structures using toluidine blue O stain. Blight symptoms by Xcs were reported to be due to the blockage of nutrients and water flow. Psse showed thining and disruption of mesophyll tissues on the appearance of chlorotic symptoms 3-4 DAI. There were only empty spaces of tissues were observed 7 DAI. Overall the infection was same but delayed in tolerant genotypes. Disruption of mesophyll tissues might be due to the action of chlorosis producing toxin (coronatine) that degraded chloroplast membrane of host tissues. The forth study was conducted to detect the virulence factors of Xcs and Psse using suitables bioassays such as antibacterial test, induction of potato hypertrophic outgrowth and seedlings assay. Xcs and Psse (chl-) isolates showed zone of inhibition. The zone of inhibition produced by chl- isolates showed that chl- was not the defective mutant of chl+ isolates as reported in third study, but this test confirmed that these isolates produced another class of toxin that showed antibacterial activity. Induction of hypertrophic outgrowth in potato tuber and seedlings inhibition from culture filtrate of chl+ isolates of Psse confirmed that the toxin produced by these isolates was similar to phytotoxin coronatine (a polyketide molecule) and it might mimics the action of one of the phytohormones. The fifth study was conducted to extract the virulence factors as well as their purification and identification was also performed. Identification was made on the basis of reference data. Crude extracts of acetone preparation of Xcs and Psse (chl-) isolates were concentrated on silica TLC plates. Further purification was carried out by HPLC and TLC. The toxic aciticity eluted from the HPLC column after 10 min corresponding with single active peak showed antibacterial activity. Reverse phase HPLC of chl- isolates extracted partially purified produced an elution pattern like reported in mangotoxin from Pss strain UMAF0158. Acetone praperation of cell free culture filtrates of virulent Xcs also showed active peaks having phytotoxic activity obtained from the HPLC column after 10 min.
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