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معافی دا پھل

معافی دا پھل

سکولاں وچ گرمی دیاں چھٹیاں سن، عادل اپنی دادی نوں ملن ملتان آیا ہویا سی۔ اوہ چھٹیاں وچ اپنی دادی کول ضرور آندا سی۔ ایس لئی کہ اوس دی دادی اوس نوںبہت پیار کر دے تے کئی شیواں لے کے دیندی سی۔ اک وار دادی نے اوس نوں آکھیا کہ میں بازار توں کجھ گھر دیاں شیواں خریدنیاں نیں۔ توں میرے نال بازار چل۔ عادل تیار ہو گیا۔ دونویں اک رکشے وچ بیٹھے تے بازار آ گئے۔

بازار وچ آ کے پہلاں اوہناں کجھ چیزاں دیاں قیمتاں پچھیاں تے فیر اک وڈے سٹور اندر چلے گئے۔ ایس سٹور تے پھل، سبزیاں، بسکٹ، مٹھائی کپڑے، بالاں دے کھڈوانے تے ضرورت دی ہر شے موجود سی۔ دادی خریداری وچ مصروف ہو گئی تے عادل اوتھے موجود شیواں نوں ویکھن لگ پیا۔ مٹھائی والے کائونٹر اگوں لنگھدے ہویاں۔ اوس دا مٹھائی کھاون نوں دل کیتا۔ اوس ادھر اُدھر ویکھیا۔ جدوں اوس نوں یقین ہو گیا کہ اوس نوں کوئی نئیں ویکھ رہیا تاں اوس اک گلاب جامن چک کے کھا لیا۔ جدوں اوس دی دادی مٹھائی والے کائونٹر کولوں لنگھی تاں عادل اوتھے ای کھڑا منہ ہلا رہیا سی تے شیرہ اوس دے منہ اتے لگا ہویا سی۔ اوس دی دادی فوراً اوس سٹور توں باہر لے کے آئی تے پچھیا کہ توں کیہ کھاہدا اے؟ اوس جواب دتا کجھ وی نئیں۔ دادی نے پچھیا کیہ توں مٹھائی کھاہدی اے؟ اوس جواب دتا جی دادی اماں، دادی اماں نے پچھیا کیہ ایس سٹور توں چک کے کھاہدی اے؟ اوس آکھیا جی ہاں۔ دادی نے پچھیا توں سٹور والے دی اجازت توں بغیر کھاہدی اے؟ اوس آکھیا جی ہاں، دادی نے آکھیا جا کے سٹور والے کولوں معافی منگ تے نالے پیسے ادا کر۔ عادل کیہندا اے کہ جے میں اوس نوں...

Work-family conflict and fear of COVID-19 and its relationship with the physical and mental health of Pakistani working women

Work-family conflict is a conflict between societal expectations and the interaction of interrelated work and family domains. It is essential to understand the impact of COVID-19 on working women's physical and mental health in Pakistan to advise better health policy. This research aims to determine work-family conflict’s impact on working women’s physical and mental health. Through non-probability sampling, 100 working women were sampled online across different cities of Pakistan. The participants were selected from different working fields. Only women above the age of 20 years were eligible for participation in this study. The study found that work-family conflict positively correlates with the fear of COVID19, blood pressure, depression, and anxiety symptoms. Psychologists, counselors, and general physicians for primary and preventive care in Pakistan need to work towards counselling and supporting the health needs of working women to prevent biopsychosocial problems related to work-family conflict and fear of COVID-19. Keywords: anxiety, blood pressure, depression, fear of covid-19, work-family conflict.

Molecular Studies on Plant Based Cyclotides for Protein-Protein Interaction

Bioactive agents like secondary metabolites and peptides are gaining much interest while addressing the issues of agricultural and health threats including pests and pathogens and in drug development. Among the plant bioactive peptides, cyclotides being disulphide rich, stable, resistant and having ability to graft epitopes on it or allow sequence variations in its different loops that are very important regarding biological application and research interests for drug development and delivery. The present study was therefore focused on evaluating the bioactive potential of cyclotide bearing indigenous plants including Viola odorata, Viola tricolor, Viola hybrid, Petunia, Clitoria ternatea, pansy F1, Panicum vigatum, Panicum laxam, Panicum maximum and Hamelia patens. The extracts were prepared in phosphate buffer saline (PBS) and protein extraction buffer (PEB). Ptunia and MCOTI-I both separately showed highest DPPH activity (antioxidant) due toas the activity was reduced significantly after treating with Proteinase K. Hamelia possessed highest reducing power, hemolytic, thromobolytic and antimicrobial activites. Mutagenic responses of the (Ames test) medicinal plants were not significant. DNA demage protection assays were also done on all protein extracts including MCOTI-I. Cyclotide genes were also isolated from the selected plant’s DNA using specific primers. It was found that the chimeric arrangement of cliotide gene (from Clitoria plant) as most attractive with conserved sequences in cyclotide and Albumin-1 domains and little intronic variations. Moreover, for cyclotide-protein interaction studies, Texas Red-DBCO amine dye was synthesized to label MCOTI-I-AziF with p-azidophenylalanine Uaa in origami DE3. Optimizations were first done by using the MCOTI-I and its label form MCOTI-I-Lys-TxRd. The click reaction to bind TxRd-DBCO with MCOTI-I-AziF was finally done in buffered guianidinum HCl at a molar ratio of 1:100 and analysed through LC/MS and MCOTI-I-AziF-TxRd-DBCO was confirmed by mass spectrometry. For binding assay FRET analysis was done with Trypsin-EGFP, saturation was achieved with KD value 29.7 ± 1.08 nM. Optimization of same amount of MCOTI-I inteins (using westernblot) was taken in 3-4 h. The IPTG induction avoided the overnight incubations and unwanted backgrounds. The MCOTI-I a cyclotide was expressed for the first time in yeast cells. Current research opens new understandings towards the bioactive xii potential of cyclotide bearing plants with/without peptide content, genetics of cyclotides genes from indegenous plants, optimizations of expression and in vitro labeling studies of MCOTI-I for optical studies regarding drug development and targeting.
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