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باب پنجم: پہاڑ وں کا مطالعہ

پتھریلی اور اونچی جگہ کے لئے پہاڑ کی اصطلاح مستعمل ہے۔ پہاڑ دراصل سنسکرت زبان کا لفظ ہے، اردو میں اس کے متبادل "کوہ، پربت اور جبل " مستعمل ہیں۔ پہاڑ دنیا میں زمین کی خشک سطح کا پانچواں حصہ ہیں۔ پہاڑ دنیا کی آبادی کے دسویں حصے کو گھر مہیا کرنے کا وسیلہ ہیں۔ دلچسپ امر یہ ہے کہ دنیا میں 80 فیصد پینے کا پانی انہی پہاڑوں میں سے نکلتا ہے۔

11 دسمبر کو پہاڑوں کا عالمی دن منا یا جاتا ہے۔ یہ عالمی دن منانے کا اصل مقصد یہ ہے کہ دنیا کو ماحولیاتی خطرات سے بچانا، قدرتی ماحول کو برقرار رکھنا، ماحولیاتی آلودگی سے بچاؤ اور پہاڑوں کے قدرتی حسن کو برقرار رکھنے کے لیے اقدامات کا شعور اجاگر کرنا ہے۔ پہاڑ عام طور پر پہاڑی سے بلند اور دشوار گزار ہوتا ہے، پہاڑوں کے مطالعہ کے علم کو "اوروگرافی" کہا جا تا ہے۔ دنیا کی دوسری بلندترین چوٹی 'کے۔ ٹو'پاکستان میں واقع ہے، جس کی بلندی 8611 میٹر ہے۔ دنیا کی 8000میٹر سے بلند چوٹیوں میں سے پانچ چوٹیاں پاکستان میں ہیں۔ دنیا کی نویں بلند ترین چوٹی نانگا پربت ہے، جس کی بلندی 8126 میٹر ہے۔

زمین کا توازن (Balance of Earth)

قرآن مجید میں بیشتر مقامات پر فرمایا گیا ہے کہ پہاڑ زمین کی مضبوطی کے لیے زمین میں میخوں کی طرح گاڑے گئے ہیں۔

قرآن پاک میں ہے:

"وَجَعَلْنَا فِي الْاَرْضِ رَوَاسِيَ اَنْ تَمِيْدَ بِهِمْ "[1]    

" اور ہم نے زمین میں پہاڑ جما دیے تاکہ وہ انہیں لے کر ڈھلک نہ جائے۔ "

زمین پر پہاڑوں کو نصب کرنے کا مقصد یہ ہے کہ زمین ڈھلکنے اور جھتکے لگنے سے...

Detection of Metalo-Beta-Lactamase Gene in Carbapenem Resistant Pseudomonas Aeruginosa Isolated From Lahore, Pakistan Detection of Metalo-Beta-Lactamase Gene in Pseudomonas Aeruginosa

Pseudomonas aeruginosa is a widespread organism, caused severe nosocomial infection in human and associated with multiple drug resistance (MDR)Objective: The present study was carried out to observe current antimicrobial resistant pattern of Pseudomonas aeruginosa in Lahore and to detect the Metallo-beta-lactamase (MBL) gene in carbapenem resistantPseudomonas aeruginosaMethods: By screening 360 samples total 123 Pseudomonas aeruginosa was identified by standard microbiology techniques such as microscopy and biochemical testing. The isolated Pseudomonas aeruginosa was evaluated for drug resistance by disc diffusion method and polymerase chain reaction(PCR) was used to identify the carbapenem resistance causing gene (bla-VIM and bla-IMP) Results: Following antibiotic resistant pattern was observed, Gentamycin (59.00%), Ceftazidime(58.7%), Ceftriaxone (58.00%), Cefotazime (57.0%) and Ciprofloxacin (55.00%). Resistance rates to carbapenem group of antibiotics is Doripenem (30.5%) Meropenem(31.0%) and Imipenem (28.0%). Out of 123 samples of Pseudomonas aeruginosa, 28 isolates were found resistant to carbapenem group of antibiotic which was supposed to be highly sensitive for this bacterium. Molecular based identification of resistance genes showed that bla-IMP gene was present in 32.1% (09) and bla-VIM was found positive in 17.8% (04) samples. Metallo-beta-lactamasesproducing genes (bla-VIM and bla-IMP), amongcarbapenem resistant Pseudomonas aeruginosa were detectedin 28.1% of samples. If other carbapenem resistant gene were also included this number might be higherConclusions: PCRbased test should be included in routine laboratory examination for quick detection of the resistancecausing genes.

Extraction of Bioethanol from Agricultural Wastes by Using Termites Gut Flora Through Fermentation

Pakistan utilizes huge amount of budget to import petroleum products from other countries because very limited resources of fossil fuels are available in country. However combustion of fossil fuel increases concentration level of greenhouse gases, those are destroying environment. There is a need of some alternate and cheaper resources of energy to overcome the deficiency of fuels. There are number of challenges for the marketable applications of the advanced technologies of fuel production which includes pretreatment methods and enzymes production. For the microbiologist and ecologist, termite gut represents an outstanding representative of highly structured micro environments and therefore also called as mini biorefineries. Because the decomposition of lignocelluloses is proficiently perform by these soil insects. A study was conducted to develop a method for the production of bioethanol from agricultural waste materials which includes corn stover, cotton stalk and rice straw by using cellulolytic bacteria from termite gut. Bacteria were isolated from termite gut and identified by 16S rRNA gene sequencing. The isolates were screened for CMCase and xylanase activity. Three isolates were selected among which two were bacillus sp. and the one was cellulomonas sp. Their avcilase CMCase and xylanase activities were studied on selected temperature and pH. It was found that all of the bacterial isolates have shown higher avicelase activity at 40 oC, CMCase and xylanase activity at 50 oC. Isolate 9x, 10 and 31 have shown higher avicelase activity at 7 pH, whereas CMCase activity was found to be optimum at pH 8. In case of xylanase activity isolate 9x and 10 showed higher activity at pH 6 and isolate 31 at pH 7. Biological and chemical pretreatments were compared for corn stover, cotton stalk and rice straw. Efficiency of enzymes of bacterial isolates for saccharification of agricultural substrates was assessed. It was observed that xylanase (isolate 9x) 1 2 released more sugar amount from corn stover as compared to other substrates. Direct saccharification and fermentation of corn stover and rice straw was carried out using all three bacterial isolates without using pretreatment methods. Ethanol concentration was analyzed by using High performance Liquid chromatography. It was observed that isolate 31 has produced higher amount of ethanol by using corn stover. Therefore it was found that these bacterial enzymes have the potential to hydrolyze not only pure substrates but can also degrade agricultural substrates. Bacterial isolates obtained from termite gut have potential to can convert different agricultural substrates into valuable bioproducts including various alcohols (ethanol) which may have new innovative source for producers.
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