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بسمل سعیدی
بسمل سعیدی
افسوس ہے پچھلے دنوں جناب بسمل سعیدی کاچند ماہ کی مسلسل علالت کے بعد دہلی میں انتقال ہوگیا۔مرحوم ٹونک(راجستھان)کے خاندان سادات سے تعلق رکھتے تھے، اس خاندان کی قرابت حضرت سید احمد شہید سے بھی تھی۔اس خاندان میں تعلیم قدیم وتعلیم جدید دونوں کے نامور افرادواشخاص پیداہوئے۔ مرحوم بلند پایہ شاعر اورصاحب فن استاد سخن تھے، غزل اورنظم دونوں پریکساں قدرت تھی، اُن کے فیض صحبت سے سینکڑوں نوجوان بڑے شاعر ہوگئے۔ایک عرصہ سے دہلی میں مقیم تھے۔خاندانی اعتبار سے صاحب املاک وجائیداد تھے لیکن ایک ایسی افتاد پڑی کہ سب کچھ جاتارہا۔طبعاً نہایت غیور وخوددار تھے۔ زندگی کے آخری سال عسرت اورتنگدستی میں گزرے یہاں تک کہ جسم وجان کا رشتہ بھی اسی عالم میں منقطع ہوگیا۔اﷲ تعالیٰ مغفرت وبخشش کی نعمتوں سے نوازے۔آمین
[ستمبر۱۹۷۷ء]
USAGE OF INFORMATION AND COMMUNICATION TECHNOLOGY TOOLS IN COURTS AND ITS IMPLICATIONS: A CASE STUDY OF ISLAMABAD HIGH COURT
John Rawls, an American Political Philosopher worked on the very idea of justice in his writings throughout his life. He was of the opinion that an institution is just and fair only when its principles and rules are prepared to benefit all the members of the society equally and if there is an unequal distribution then it must advantage all of them especially the ones who are least i.e. Needy. The political institutions must shape up such kind of policies in order for the development of the wellbeing of the individuals in the society by providing them with resources, opportunities, basic necessities etc. Equally. Whereas, the conception of social justice is also based on equality and the human rights where they could be treated as free and equals i.e. An egalitarian society. In this research paper we will analyze the significance of John Rawl’s theoryGenetic Transformation and Withanolides Analysis of Withania Coagulans Dunal .
Withania coagulans is a valuable medicinal plant that holds a key place in folk medicine due to its extensive use in the treatment of many ailments. Biological activities like antimicrobial, anti-inflammatory, antitumor, hepatoprotective, antihyperglycemic, immunosuppressive, and antitumor, free radical scavenging and anti-depressant have been attributed to the plant due to the presence of secondary metabolites. Withanolides are the major class of secondary metabolites found in W. coagulans. In the present study, bioactive withanolides of Withania coagulans collected from 11 different sites in Pakistan were analyzed. Total withanolide concentration differed by ~17-fold between sites, ranging from 1.01 ± 0.01 mg/g dry weight (mean ± SE) at Jand to 16.83 ± 0.02 mg/g at Mohmand Agency. Different tissues varied in their total withanolide content and there were strong inverse correlations between site annual precipitation versus withanolide amounts in fruits (r = -0.84, P = 0.001), leaves (r = -0.88, P< 0.001), roots (r = -0.91, P< 0.001), and total (r = -0.89, P< 0.001), but not stems (r = -0.20, P = 0.556). Leaf extracts of plants collected from Mianwali and Mohmand Agency possessed high anti-cancer activity in terms of increased induction of apoptosis and decreased cell viability, cell proliferation, cell invasion, and cell migration of different prostate cancer cell lines. These results are useful for the selection of withanolide rich germplasm with potent anti-cancer properties. Owing to the therapeutic importance of these secondary metabolites in W. coagulans, it would be useful to enhance its withanolide content. Therefore, W. coagulans was transformed with the rol A gene which is known to enhance secondary metabolites. Prior to producing transgenic plants, an effective protocol for in vitro regeneration was developed. Maximum embryogenic callus induction response was observed at MS(Murashige and Skooj) enriched with 4.0 μM naphthalene acetic acid and 6.0 μM benzyl adenine, whereas maximum shoot formation response (100%) was observed at 8 μM benzyl adenine. Elongated shoots demonstrated good in vitro rooting (80%) with 2.5 μM indole butyric acid. To produce transgenic plants, pre-culturing of explants was done for 3 days. The GV3101 strain of A. tumefaciens, containing pCV002-A vector harboring the rolA gene of Agrobacterium rhizogenes, was used for infection for 48 hours. Three independent transgenic lines were generated that showed stable integration of the transgene confirmed by PCR analysis. These transgenic lines were selected and screened for eight biologically active withanolides using ultra-highpressure liquid chromatography (UHPLC) with mass spectrometry (MS). Results revealed that transformed lines possessed higher withanolide content as compared to untransformed ones. Extracts of these transgenic lines, which possessed higher levels of total withanolide content, were tested for bioactivity against different prostate cancer cell lines. The results showed that transformed plants extracts had higher anticancer activity in terms of decreased cell viability of prostate cancer cell lines. The data presented could be useful for the selection of potent and withanolide-rich germplasm from natural populations or transformed cell culture.Journals by Discipline
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