ڈاکٹر تارا چند
گذشتہ اکتوبر میں ڈاکٹر تاراچند کے انتقال پر ملال سے بھی ہندوستان میں ایک بڑا علمی خلاء پیدا ہوگیا، وہ الہ آباد کے کایستھ پاٹ شالہ کی مدرسی اور پرنسپلی کے بعد الہ آباد یونیورسٹی کے وائس چانسلر بھی ہوئے، پھر حکومت ہند کے تعلیمی مشیر بنے، ایران میں ہندوستان کی طرف سے سفیر بناکر بھیجے گئے، وہاں سے واپسی پر پارلیمنٹ کے رکن نامزد کئے گئے لیکن ان کی اصلی شہرت اور مقبولیت ایک بلند پایہ مورخ کی حیثیت سے ہوئی، انھوں نے ’’انفلوانس آف اسلام ان انڈین کلچر‘‘ پر ایک مقالہ لکھ کر آکسفورڈ سے ڈاکٹریٹ کی ڈگری حاصل کی، یہ ان کی ابتدائی تحقیقی کوشش تھی، لیکن اس سے بہتر کتاب اس موضوع پر اب تک نہیں نکلی، آخر میں وہ ہسٹری آف دی فریڈم موومنٹ کے بورڈ کے اڈیٹر تھے، ان کی ادارت میں یہ کام بڑی خوش اسلوبی سے انجام پاتا رہا، ان کی تحقیقی تحریروں میں دیدہ دری کے ساتھ بڑی فراخدلی بھی ہوتی، وہ ہندی اردو کی حسین آمیزش یعنی ہندوستانی زبان کے بھی بڑے موئد تھے، اس کے کچھ اچھے نمونے بھی لکھ کر پیش کرتے رہے، ان کی نیک نامی اور مورخانہ بصیرت کی وجہ سے ان کی خوشگوار یادوں کا چراغ علمی حلقہ میں برابر روشن رہے گا۔ (’’ص ، ع‘‘، نومبر ۱۹۷۳ء)
According to Qur’an, the difference of opinion among peoples of the world is natural and something that will always be there. However, in order to stop the difference from becoming a conflict, people should hold dialogue. The significance of dialogue in Islam is well understood by the fact that God chose to hold dialogue with angels concerning the creation of man. Furthermore, the Qur’an declares dialogue the greater jihad and arrangement of a successful dialogue is considered as a manifest victory In order to arrange a successful dialogue, Qur’an lays out a number of principles: 1- Dialogue should be held in such a nice way that it may lead the opponent to get a close friend. For this it is necessary to speak mildly and the dialogue must be based on wisdom and sincerity. 2- Dialogue should rest on the principle of mutual respect and should not contain any kind of abusive and taunting language. 3- Dialogue must not override the principle of justice and equality and must not be affected by the past experiences or personal grievances towards the opponent. 4- Dialogue should not address the issue of pulling everyone together, e.g. The opponent (for example a nation) should not be blamed for the evil deeds of few. 5- Dialogue should be held with an attitude that is characterized by patience and tolerance and efforts must be made to keep the vicious elements out from harming the process. 6- Both parties should openly acknowledge and recognize the mutually positive attributes. 7- Imposing one’s opinions upon the opponent must not be the objective of dialogue. 8- Both parties should, despite the inherent difference of opinion, pursue to find practical solutions by striving towards finding a common ground.
Medicinal plants were playing an important role in our lives due to their remedial properties.
Lifesaving medicines were produced by these plants. For their better use in food and medicines
genomes sequencing of these medicinal plants was needed. Ajuga bracteosa is a medicinal plant
in family Lamiaceae (mint family). It is a herbal plant and used in many medicines. In this
research work chloroplast genome sequencing of Ajuga bracteosa was carried out by next
generation sequencing technique. DNeasy plant DNA extraction kit was used for the extraction of
DNA from fresh young leaves of Ajuga bracteosa. Quality and quantity of DNA was evaluated
using agarose gel electrophoresis and uDrop plate of Multiskan GO spectrophotometer. DNA was
lyophilized before shipment to Novagene, Hong Kong, a commercial NGS facility. Sequencing
was done using Illumina HiSeq 2500 instrument, using PE150 run, and approx. 4Gb data was
produced. Quality of short reads was checked using SolexaQA tool. Short reads were mapped
back to Ajuga reptans chloroplast genome downloaded from NCBI. Mapping was visualized
using Tablet tool, which attested to high coverage depth of chloroplast genome. The genome was
de novo assembled using Velvet tool, and several velvet runs were performed by changing kmer
values. Assembled contigs from these Velvet runs were imported in Geneious tool and used to
generate full length chloroplast genome. The assembled genome size was 150,373 bp, divided in
usual LSC, SSC and IRs. Overall genome structure and gene synteny matched to Ajuga reptans
chloroplast genome. Phylogenetic analysis showed divergence of Ajuga bracteosa from its
relatives in family Lamiaceae. This study showed intra specific and interspecific relationship
within family Lamiaceae. So, this work will help the researchers in proper identification and
differentiation of Ajuga bracteosa from the other plants of same family.