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آپؐ کے نقشِ پا سے مزین زمیں ، خاتم المرسلیں

آپؐ کے نقشِ پا سے مزین زمیں، خاتم لمرسلیںؐ!
اَرضِ بطحا نے پائے ہیں کیسے نگیں، خاتم لمرسلیںؐ!

آپؐ کی بات کا صدق دل سے یقیں ،اے رسولِ امیں!ؐ
آپؐ کو غیر کہتے ہیں صادق امیں ،خاتم لمرسلیںؐ!

آپؐ کو جو رکھیںجان و دل کے قریں ،رحمتِ عالمیں!ؐ
صاف کہتا ہے قرآں انہیں مومنیں ، خاتم لمرسلیںؐ!

آپؐ کا جو ہوا ، سب اُسی کا ہوا ، اے حبیب خداؐ!
آپؐ کا جو نہیں ، وہ کہیں کا نہیں ، خاتم لمرسلیںؐ!

خوش طبع ، خوش ادا ، سیرت خوش صفا ، صورتِ والضحیٰ
خوش خط و خوبرو ، دلکش و دلنشیں ، خاتم لمرسلیںؐ

آپؐ کے بعد آئے نبیؐ دُوسرا ؛ میں نہیں مانتا
سیّد الاوّلیں ، سیّد الآخریں ، خاتم لمرسلیںؐ!

تُو لکھے حرفِ مدحت، پڑھے نعت کیا ، تیری اوقات کیا
دی گئے تجھ کو عرفانؔ! لحنِ حسیں ، خاتم لمرسلیںؐ

Peranan Customer Service Terhadap Pelayanan Jasa Kredit

Keberadaan seorang customer service sangat diperlukan didalam organisasi perusahaan, agar dapat berfungsi sebagai fungsi manajemen modern yang mempunyai fungsi melekat pada manajemen perusahaan, yakni bagaimana berperan dalam melakukan komunikasi timbal balik untuk tujuan menciptakan saling pengertian, saling menghargai, saling mempercayai menciptakan goodwill, memperoleh dukungan masyarakat dan sebagainya demi tercapainya citra yang positif bagi suatu perusahaan. Perkembangan masyarakat dewasa ini menghendaki agar tiap-tiap perusahaan perlu mengatur hubungannya melalui pelayanan dengan berbagai keputusan masyarakat agar tercapai hubungan yang serasi dan harmonis. Penelitian ini bertujuan untuk mengetahui dan menganalisis peranan customer service Terhadap Pelayanan Jasa Kredit Pada PT. Pegadaian Cabang Gunungsitoli. Sampel penelitian ini diambil dengan menggunakan metode random sampling (sampling acak). Penelitian ini menggunakan pendekatan kualitatif yang datanya diolah melalui tiga jalur analisis data kualitatif yaitu reduksi data, penyajian data dan penarikan kesimpulan.

Preparation and Characterization of Modified Streptokinase from Streptococcus Spp.

Streptokinase (SK) is a fibrinolytic protein which has been produced by several species of β-hemolytic Streptococci. It has been extensively used to treat cardiovascular disorders by activating plasminogen into plasmin and eventually dissolving the blood clot. The objective of the current research project was to exploit indigenously isolated Streptococcus agalactiae EBL-20 for the production of SK and enhancing its productivity by Ultraviolet (UV) irradiation and ethyl methanesulfonate (EMS) induced mutations, optimized culture conditions and nutritional requirements. Four mutants namely, S. agalactiae EBL-31, S. agalactiae EBL-32, S. agalactiae EBL-33 and S. agalactiae EBL-34 were selected by different screening procedures using triton X-100, casein plasminogen overlay assay and clot lysis assay. The optimum levels of four process variables (pH, temperature, incubation time and inoculum size) were obtained by Response Surface Methodology (RSM). According to statistical results, a temperature value of 37.5⁰C and pH 7 of the fermentation medium with 2.50 mL inoculum size for 36 hours of incubation was considered as optimum for enhanced SK production. After optimization, mutant derived strains EBL-31, EBL-32, EBL-33 and EBL-34 resulted in 2.01, 2.30, 3.70 and 2.48 fold increased activity as compared to parent S. agalactiae EBL-20, respectively. The best mutant obtained after performing optimization studies was found to be S. agalactiae EBL-33 with 3.70 fold increased activity, hence it was purified and exploited for characterization studies with reference to S. agalactiae EBL-20.The purification of SK by DEAE-cellulose and Sephadex-G100 resulted into specific activity of 447.6 and 1278.87 U mg⁻¹ from parent S. agalactiae EBL-20 and mutant derived S. agalactiae EBL-33, respectively. The molecular weight of SK obtained from both the strains of S. agalactiae was found to be 47 kDa. Study of kinetic and thermodynamic parameters revealed that SK obtained from S. agalactiae EBL-33 has more potential for utilizing its substrate i.e., plasminogen with high thermostability, in contrast to SK derived from S. agalactiae EBL-20. The study concludes that both UV rays and EMS are effective mutagenic agents for enhanced production of SK from S. agalactiae. Moreover, enhanced SK yield can be obtained by optimizing the nutritional necessities and fermentation/cultivation conditions of the microorganism.
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